Version 1
: Received: 2 September 2024 / Approved: 3 September 2024 / Online: 5 September 2024 (06:12:56 CEST)
How to cite:
Serio, B.; Muller, J.; Silva, M.; Figueiredo, F.; Martins, D. Phytochemistry and Evaluation of the Anti-inflammatory Activity of the Hydroethanolic Extract of Virola elongata (Benth.) Warb. Stem Bark. Preprints2024, 2024090265. https://doi.org/10.20944/preprints202409.0265.v1
Serio, B.; Muller, J.; Silva, M.; Figueiredo, F.; Martins, D. Phytochemistry and Evaluation of the Anti-inflammatory Activity of the Hydroethanolic Extract of Virola elongata (Benth.) Warb. Stem Bark. Preprints 2024, 2024090265. https://doi.org/10.20944/preprints202409.0265.v1
Serio, B.; Muller, J.; Silva, M.; Figueiredo, F.; Martins, D. Phytochemistry and Evaluation of the Anti-inflammatory Activity of the Hydroethanolic Extract of Virola elongata (Benth.) Warb. Stem Bark. Preprints2024, 2024090265. https://doi.org/10.20944/preprints202409.0265.v1
APA Style
Serio, B., Muller, J., Silva, M., Figueiredo, F., & Martins, D. (2024). Phytochemistry and Evaluation of the Anti-inflammatory Activity of the Hydroethanolic Extract of Virola elongata (Benth.) Warb. Stem Bark. Preprints. https://doi.org/10.20944/preprints202409.0265.v1
Chicago/Turabian Style
Serio, B., Fabiana Figueiredo and Domingos Martins. 2024 "Phytochemistry and Evaluation of the Anti-inflammatory Activity of the Hydroethanolic Extract of Virola elongata (Benth.) Warb. Stem Bark" Preprints. https://doi.org/10.20944/preprints202409.0265.v1
Abstract
Background: Previous studies of the hydroethanolic extract of Virola elongata inner stem bark (HEVe) have demonstrated its antioxidant, gastroprotective, and antiulcer properties, but have not evaluate its anti-inflammatory potential. Methods: HEVe was obtained by maceration and phytochemicallly analyzed. Its systemic anti-inflammatory activity was assessed by its effect on lipopolysaccharide (LPS)-induced peritonitis in mice. HEVe gel (HEgVe) was employed to evaluate topical anti-inflammatory activity by measuring the ear edema resulted from croton oil-induced dermatitis in mice. Cell viability assay was conducted to determine non-cytotoxic concentrations of HEVe. RAW 264.7 cells were stimulated by LPS to determinate cytokine and nitric oxide production.Results: Phytochemical analysis of HEVe revealed the presence of phenolic acids, neolignans, flavonoids, and monomeric catechins. Oral treatment of acute peritonitis with HEVe reduced total leukocytes, neutrophils, TNF-α and IL-1β, and elevated IL-10 levels. The application of HEgVe reduced local edema. The HEVe on RAW 264.7 cells exhibited no cytotoxicity and cells with HEVe reduced TNF-α, IL-1β, and NO levels, and increased IL-13 levels. Conclusions: HEVe demonstrated systemic and topical multitarget anti-inflammatory activity, which is likely due to the combined effects of secondary metabolites. HEVe emerges as a promising herbal remedy for inflammation with minimal cytotoxicity, emphasizing its potential therapeutic significance.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
