Version 1
: Received: 3 September 2024 / Approved: 4 September 2024 / Online: 4 September 2024 (08:26:00 CEST)
How to cite:
Ali, B.; Chauhan, A.; Kumar, M.; Kumar, P.; Carolus, H.; Lobo Romero, C.; Vergauwen, R.; Singh, A.; Rudramurthy, S. M.; Van Dijck, P.; Ibrahim, A. S.; Prasad, R. A Comprehensive Analysis of the Lipidomic Signatures in Rhizopus delemar. Preprints2024, 2024090325. https://doi.org/10.20944/preprints202409.0325.v1
Ali, B.; Chauhan, A.; Kumar, M.; Kumar, P.; Carolus, H.; Lobo Romero, C.; Vergauwen, R.; Singh, A.; Rudramurthy, S. M.; Van Dijck, P.; Ibrahim, A. S.; Prasad, R. A Comprehensive Analysis of the Lipidomic Signatures in Rhizopus delemar. Preprints 2024, 2024090325. https://doi.org/10.20944/preprints202409.0325.v1
Ali, B.; Chauhan, A.; Kumar, M.; Kumar, P.; Carolus, H.; Lobo Romero, C.; Vergauwen, R.; Singh, A.; Rudramurthy, S. M.; Van Dijck, P.; Ibrahim, A. S.; Prasad, R. A Comprehensive Analysis of the Lipidomic Signatures in Rhizopus delemar. Preprints2024, 2024090325. https://doi.org/10.20944/preprints202409.0325.v1
APA Style
Ali, B., Chauhan, A., Kumar, M., Kumar, P., Carolus, H., Lobo Romero, C., Vergauwen, R., Singh, A., Rudramurthy, S. M., Van Dijck, P., Ibrahim, A. S., & Prasad, R. (2024). A Comprehensive Analysis of the Lipidomic Signatures in <em>Rhizopus delemar</em>. Preprints. https://doi.org/10.20944/preprints202409.0325.v1
Chicago/Turabian Style
Ali, B., Ashraf S Ibrahim and Rajendra Prasad. 2024 "A Comprehensive Analysis of the Lipidomic Signatures in <em>Rhizopus delemar</em>" Preprints. https://doi.org/10.20944/preprints202409.0325.v1
Abstract
Certain species of Mucorales have been identified as causative agents of mucormycosis, a rare yet often lethal fungal infection. Notably, these fungi exhibit intrinsic resistance to common azole drugs, which target lipids. Given the pivotal role of lipids in drug resistance and their contribution to the innate resistance to azoles, this study provides a comprehensive overview of key lipid classes, including sphingolipids (SLs), glycerophospholipids(GPLs), and sterols, in Rhizopus delemar 99-880, a well-characterized reference strain among Mucorales. Through high-throughput analysis involving the identification and quantification of intermediates, we have elucidated the biosynthetic pathways of SLs, PGLs, and sterols. Notably, the acidic SLs were not found probably because the acidic branch of the SL biosynthesis pathway terminates at α-hydroxy phytoceramides as evident by their high abundance. Intermediates in the neutral SL pathway incorporated higher levels of 16:0 fatty acid compared to other pathogenic fungi. A strikingly high phosphatidylethanolamine (PE)/phosphatidylcholine (PC) ratio was observed among GPLs. Ergosterol remains the major sterol, similar to other fungi, and our analysis confirms the existence of alternate ergosterol biosynthesis pathways. The total lipidomic profile of R. delemar 99-880 offers insights into its lipid metabolism and potential implications for studying pathogenesis and drug resistance mechanisms.
Keywords
mucormycosis; drug resistance; posaconazole; amphotericin B; sphingolipids; phospholipids; sterols; mass-spectrometry
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
