Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics

Ruth Chinyere Njoku; Marianna Martinelli; Chiara Giubbi; Sofia De Marco; Barbara Torsello; Morena D'Avenia; Manuela Sironi; Cristina Bianchi; Clementina Elvezia Cocuzza

doi:10.20944/preprints202409.0376.v1

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preprints.org > biology and life sciences > virology > doi: 10.20944/preprints202409.0376.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics

^* ,

Clementina Elvezia Cocuzza

Version 1 : Received: 3 September 2024 / Approved: 4 September 2024 / Online: 5 September 2024 (00:26:00 CEST)

How to cite: Njoku, R. C.; Martinelli, M.; Giubbi, C.; De Marco, S.; Torsello, B.; D'Avenia, M.; Sironi, M.; Bianchi, C.; Cocuzza, C. E. Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics. Preprints 2024, 2024090376. https://doi.org/10.20944/preprints202409.0376.v1 Njoku, R. C.; Martinelli, M.; Giubbi, C.; De Marco, S.; Torsello, B.; D'Avenia, M.; Sironi, M.; Bianchi, C.; Cocuzza, C. E. Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics. Preprints 2024, 2024090376. https://doi.org/10.20944/preprints202409.0376.v1

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MDPI and ACS Style

Njoku, R. C.; Martinelli, M.; Giubbi, C.; De Marco, S.; Torsello, B.; D'Avenia, M.; Sironi, M.; Bianchi, C.; Cocuzza, C. E. Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics. Preprints 2024, 2024090376. https://doi.org/10.20944/preprints202409.0376.v1

APA Style

Njoku, R. C., Martinelli, M., Giubbi, C., De Marco, S., Torsello, B., D'Avenia, M., Sironi, M., Bianchi, C., & Cocuzza, C. E. (2024). Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics. Preprints. https://doi.org/10.20944/preprints202409.0376.v1

Chicago/Turabian Style

Njoku, R. C., Cristina Bianchi and Clementina Elvezia Cocuzza. 2024 "Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics" Preprints. https://doi.org/10.20944/preprints202409.0376.v1

Abstract

Background: Reliable HPV testing and genotyping are essential for quality assurance in HPV-based primary screening, disease management and for monitoring the impact of HPV vaccination. The clinical validation of HPV molecular diagnostic assays has significantly contributed to these objectives, however little emphasis has been placed in assuring sample quality. This study aims to assess the accuracy of sample cellularity assessment using C-C Motif Chemokine Receptor 5 (CCR5) gene target as a marker of sample adequacy in molecular diagnostics. Methods: Jurkat cell line samples were counted both using Thoma cell-counting chamber and Fluorescent-Activated Cell Sorting (FACS). Jurkat cell line samples at three different concentrations were subsequently evaluated using the OncoPredict HPV Quality Control (QC) real-time PCR assay, employing CCR5 for molecular cellularity quantification. Results: Cellularity values obtained were comparable across the 3 different methods for all dilutions of the cell line tested. Conclusions: The results obtained from this study show that CCR5 represents a promising molecular marker for the accurate quantification of sample cellularity, confirming its use as a reliable sample adequacy control, thus reducing the risk of “false negative” results.

Keywords

C-C Motif Chemokine Receptor 5 (CCR5); Human Papillomavirus (HPV); Sample Adequacy Control (SAC); OncoPredict HPV Quality Control (QC) assay

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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