Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Development and Validation of a Combined RT-LAMP Assay for the Rapid and Sensitive Detection of Dengue Virus in Clinical Samples from Colombia

Katherine Escorcia-Lindo
,
Leidy Hurtado-Gómez
,
Juan Sebastian Rosero
,
Nataly Solano Llanos
ORCID logo ,
Camilo Barrios Sánchez
,
Anderson Diaz Pérez
,
Yirys Díaz-Olmos
,
Jennifer García
,
Yesit Bello-Lemus
,
Leonardo C Pacheco-Londoño
,
Antonio J. Acosta-Hoyos
,
Lisandro A Pacheco-Lugo
* ORCID logo
Version 1 : Received: 4 September 2024 / Approved: 5 September 2024 / Online: 5 September 2024 (12:37:39 CEST)

How to cite: Escorcia-Lindo, K.; Hurtado-Gómez, L.; Rosero, J. S.; Solano Llanos, N.; Barrios Sánchez, C.; Diaz Pérez, A.; Díaz-Olmos, Y.; García, J.; Bello-Lemus, Y.; Pacheco-Londoño, L. C.; Acosta-Hoyos, A. J.; Pacheco-Lugo, L. A. Development and Validation of a Combined RT-LAMP Assay for the Rapid and Sensitive Detection of Dengue Virus in Clinical Samples from Colombia. Preprints 2024, 2024090463. https://doi.org/10.20944/preprints202409.0463.v1 Escorcia-Lindo, K.; Hurtado-Gómez, L.; Rosero, J. S.; Solano Llanos, N.; Barrios Sánchez, C.; Diaz Pérez, A.; Díaz-Olmos, Y.; García, J.; Bello-Lemus, Y.; Pacheco-Londoño, L. C.; Acosta-Hoyos, A. J.; Pacheco-Lugo, L. A. Development and Validation of a Combined RT-LAMP Assay for the Rapid and Sensitive Detection of Dengue Virus in Clinical Samples from Colombia. Preprints 2024, 2024090463. https://doi.org/10.20944/preprints202409.0463.v1

Abstract

Background: Dengue virus (DENV) infection is a significant public health concern in many trop-ical and subtropical regions, where early and rapid detection is crucial for effective patient man-agement and controlling the spread of the disease. Particularly in resource-limited, rural healthcare settings where dengue is endemic, there is a need for diagnostic methods that are both easy to perform and highly sensitive. Objective: This study focuses on the development and validation of a single-tube reverse tran-scription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of DENV. Methodology: The RT-LAMP assay designed in this work combines two sets of primers targeting the 5' and 3'-UTR regions of the dengue virus, aiming to increase the sensitivity of detection. Results: The clinical validation of this COMB-RT-LAMP in samples from febrile individuals with a serological or antigenic diagnosis showed a sensitivity of over 95%. The assay's perfor-mance was statistically compared to standard diagnostic method quantitative reverse transcrip-tion-polymerase chain reaction (qRT-PCR). Conclusions: The findings support the potential of RT-LAMP as a rapid, sensitive, and specific tool for dengue diagnosis and surveillance, particularly suitable for field use in low-resource set-tings.

Keywords

RT-LAMP; Dengue virus (DENV); Sensitivity; Point-of-Care

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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