Version 1
: Received: 6 September 2024 / Approved: 6 September 2024 / Online: 6 September 2024 (14:42:00 CEST)
How to cite:
ISBIR TURAN, A. A.; KEKLIK ŞAH, G. Innovative Surface Plasmon Resonance Aptasensor for Detecting Cocaine in Human Urine. Preprints2024, 2024090563. https://doi.org/10.20944/preprints202409.0563.v1
ISBIR TURAN, A. A.; KEKLIK ŞAH, G. Innovative Surface Plasmon Resonance Aptasensor for Detecting Cocaine in Human Urine. Preprints 2024, 2024090563. https://doi.org/10.20944/preprints202409.0563.v1
ISBIR TURAN, A. A.; KEKLIK ŞAH, G. Innovative Surface Plasmon Resonance Aptasensor for Detecting Cocaine in Human Urine. Preprints2024, 2024090563. https://doi.org/10.20944/preprints202409.0563.v1
APA Style
ISBIR TURAN, A. A., & KEKLIK ŞAH, G. (2024). Innovative Surface Plasmon Resonance Aptasensor for Detecting Cocaine in Human Urine. Preprints. https://doi.org/10.20944/preprints202409.0563.v1
Chicago/Turabian Style
ISBIR TURAN, A. A. and Gül KEKLIK ŞAH. 2024 "Innovative Surface Plasmon Resonance Aptasensor for Detecting Cocaine in Human Urine" Preprints. https://doi.org/10.20944/preprints202409.0563.v1
Abstract
This study describes the development of an optical-based surface plasmon resonance (SPR) aptasensor for the detection of cocaine. The aptasensor was prepared by first attaching gold nanoparticles to a clean SPR chip surface, followed by the addition of an aptamer to create a modified surface. This surface was characterized using Contact Angle and Atomic Force Microscopy, revealing surface roughness values of 0.28 nm and 28.12 nm for the blank and modified surfaces, respectively. The detection of cocaine was carried out in the concentration range of 1 ng/mL to 1000 ng/mL, with a detection time of approximately 8 minutes and a cocaine limit of detection (LOD) of 0.43 ng/mL. Repeatability studies were conducted, and the stability of the signal response was examined at a concentration of 200 ng/mL. Adsorption isotherm models, including Scatchard, Langmuir, and Freundlich, were calculated to assess the surface homogeneity of the SPR aptasensor chip, with the results indicating compatibility with the Langmuir isotherm model.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
