Cheng, C.-S.; Lung, F.-D. Evaluating E6 Oncoprotein and Anti-E6 Antibodies as Saliva-Based Biomarkers for HPV-Associated Oral Cancer: Insights from SPR Immunoassays. Preprints2024, 2024090680. https://doi.org/10.20944/preprints202409.0680.v1
APA Style
Cheng, C. S., & Lung, F. D. (2024). Evaluating E6 Oncoprotein and Anti-E6 Antibodies as Saliva-Based Biomarkers for HPV-Associated Oral Cancer: Insights from SPR Immunoassays. Preprints. https://doi.org/10.20944/preprints202409.0680.v1
Chicago/Turabian Style
Cheng, C. and Feng-Di Lung. 2024 "Evaluating E6 Oncoprotein and Anti-E6 Antibodies as Saliva-Based Biomarkers for HPV-Associated Oral Cancer: Insights from SPR Immunoassays" Preprints. https://doi.org/10.20944/preprints202409.0680.v1
Abstract
Background/Objectives: Human Papillomavirus (HPV)-related biomarkers, specifically E6 oncoproteins and anti-E6 antibodies, have emerged as promising indicators for detecting HPV-associated oral cavity cancers through salivary fluid analysis. This study assessed the survival outcomes of oral cancer patients who were double positive for these candidate biomarkers. Methods: Using biosensor immunoassays, we simultaneously detected E6 oncoprotein and anti-E6 antibodies in oral rinse fluids from patients with advanced T3/T4 oral cancer. Results: Preliminary findings revealed that over one-third of these patients had detectable HPV-related biomarkers in their saliva, with 37.9% testing positive for E6 oncoprotein and 31.0% showing elevated anti-E6 antibody levels. Double positivity was observed in 24.1% of cases. Although survival rate differences between the groups were not statistically significant, the double positivity subgroup demonstrated a trend toward improved survival outcomes compared to single-positive or double-negative patients. Conclusions: These initial results suggest that simultaneous detection of E6 oncoprotein and anti-E6 antibodies could be potential saliva-based biomarkers for clinical applications. Using surface plasmon resonance (SPR) techniques to identify double positivity may enhance early detection of HPV-related oral cancer, potentially informing more effective treatment strategies in clinical practice.
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
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