Version 1
: Received: 9 September 2024 / Approved: 10 September 2024 / Online: 10 September 2024 (09:00:58 CEST)
How to cite:
Trelford, C. B.; Buensuceso, A.; Tomas, E.; Valdes, Y. R.; Hovey, O.; Li, S.; Shepherd, T. G. LKB1 and STRAD Promote Epithelial Ovarian Cancer Spheroid Cell Invasion. Preprints2024, 2024090763. https://doi.org/10.20944/preprints202409.0763.v1
Trelford, C. B.; Buensuceso, A.; Tomas, E.; Valdes, Y. R.; Hovey, O.; Li, S.; Shepherd, T. G. LKB1 and STRAD Promote Epithelial Ovarian Cancer Spheroid Cell Invasion. Preprints 2024, 2024090763. https://doi.org/10.20944/preprints202409.0763.v1
Trelford, C. B.; Buensuceso, A.; Tomas, E.; Valdes, Y. R.; Hovey, O.; Li, S.; Shepherd, T. G. LKB1 and STRAD Promote Epithelial Ovarian Cancer Spheroid Cell Invasion. Preprints2024, 2024090763. https://doi.org/10.20944/preprints202409.0763.v1
APA Style
Trelford, C. B., Buensuceso, A., Tomas, E., Valdes, Y. R., Hovey, O., Li, S., & Shepherd, T. G. (2024). LKB1 and STRAD Promote Epithelial Ovarian Cancer Spheroid Cell Invasion. Preprints. https://doi.org/10.20944/preprints202409.0763.v1
Chicago/Turabian Style
Trelford, C. B., Shawn Li and Trevor G Shepherd. 2024 "LKB1 and STRAD Promote Epithelial Ovarian Cancer Spheroid Cell Invasion" Preprints. https://doi.org/10.20944/preprints202409.0763.v1
Abstract
Late-stage epithelial ovarian cancer (EOC) involves widespread dissemination of malignant disease throughout the peritoneal cavity, often accompanied by ascites. EOC metastasis relies on the formation of multicellular aggregates, called spheroids. Given that Liver Kinase B1 (LKB1) is required for EOC spheroid viability and LKB1 loss in EOC cells decreases tumor burden in mice, we investigated whether the LKB1 complex controls invasive properties of human EOC spheroids. LKB1 signalling was antagonized through CRISPR/Cas9 genetic knockout of LKB1 and/or RNAi-dependent targeting of STE20-related kinase adaptor protein (STRAD; an LKB1 activator). EOC spheroids expressing nuclear GFP (green) or mKate2 (red) constructs were embedded in Matrigel for real-time live-cell invasion monitoring. Migration and invasion were also assessed in spheroid culture using Transwell chambers, spheroid reattachment, and mesothelial clearance assays. The loss of LKB1 and STRAD signalling decreased cell invasion through Matrigel and Transwell membranes, as well as mesothelial cell clearance. In the absence of LKB1, zymographic assays identified a loss of matrix metalloproteinase (MMP) activity, whereas spheroid reattachment assays found that coating plates with fibronectin restored the invasive potential. A three-dimensional EOC organoid model demonstrated that organoid area was greatly reduced by LKB1 loss. Overall, our data indicated that LKB1 and STRAD facilitated EOC metastasis by promoting MMP activity and fibronectin expression. Given that LKB1 and STRAD are crucial for EOC metastasis, targeting LKB1 and/or STRAD could disrupt the dissemination of EOC, making inhibitors of the LKB1 pathway an alternative therapeutic strategy for EOC patients.
Keywords
Epithelial Ovarian Cancer (EOC); Liver Kinase B1 (LKB1); STE20-related Kinase Adaptor Protein (STRAD); Spheroid; Organoid; Mesothelial Clearance; Metastasis
Subject
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
