Version 1
: Received: 9 September 2024 / Approved: 10 September 2024 / Online: 10 September 2024 (15:18:57 CEST)
How to cite:
Zani, A. C. B.; de Souza, J. C.; de Andrade, A. R.; Reginatto, V. Neutral Red Film Augments Direct and Mediated Extracellular Electron Transfer Performed by Clostridium pasteurianum DSM 525. Preprints2024, 2024090776. https://doi.org/10.20944/preprints202409.0776.v1
Zani, A. C. B.; de Souza, J. C.; de Andrade, A. R.; Reginatto, V. Neutral Red Film Augments Direct and Mediated Extracellular Electron Transfer Performed by Clostridium pasteurianum DSM 525. Preprints 2024, 2024090776. https://doi.org/10.20944/preprints202409.0776.v1
Zani, A. C. B.; de Souza, J. C.; de Andrade, A. R.; Reginatto, V. Neutral Red Film Augments Direct and Mediated Extracellular Electron Transfer Performed by Clostridium pasteurianum DSM 525. Preprints2024, 2024090776. https://doi.org/10.20944/preprints202409.0776.v1
APA Style
Zani, A. C. B., de Souza, J. C., de Andrade, A. R., & Reginatto, V. (2024). Neutral Red Film Augments Direct and Mediated Extracellular Electron Transfer Performed by <em>Clostridium pasteurianum </em>DSM 525. Preprints. https://doi.org/10.20944/preprints202409.0776.v1
Chicago/Turabian Style
Zani, A. C. B., Adalgisa Rodrigues de Andrade and Valeria Reginatto. 2024 "Neutral Red Film Augments Direct and Mediated Extracellular Electron Transfer Performed by <em>Clostridium pasteurianum </em>DSM 525" Preprints. https://doi.org/10.20944/preprints202409.0776.v1
Abstract
Extracellular electron transfer (EET) is key to the success of microbial fuel cells (MFCs). Clostridium sp. often occurs in MFC anode communities, but its ability to perform EET remains controversial. We have employed Clostridium pasteurianum DSM 525 as biocatalyst in a glycerol-fed MFC, designated MFCDSM. We have also followed the EET of this biocatalyst in the presence of a mediator, namely soluble neutral red (NR), soluble methyl viologen (MV), neutral red film (FNR), or methyl viologen film (FMV). MFCDSM provided power and current densities (j) of 0.39 mW.cm-2 and 2.47 mA.cm-2, respectively, which evidenced that the biocatalyst performs direct electron transfer (DET). Introducing 150.0 µM NR or MV into the MFCDSM improved the current density by 7.0- and 3.7-fold (17.05 and 8.45 mA.cm-2), respectively. After 20 cyclic voltammetry (CV) cycles, the presence of FNR in the MFCDSM anodic chamber provided almost twofold higher current density (30.76 µA.cm-2)compared the presence of NR in the MFCDSM. Introducing MV or FMV into the MFCDSM anodic chamber gave practically the same current density after 10 CV cycles. The MFCDSM anodic electrode might interact with FMV weakly than with FNR, so FNR is more promising to enhance C. pasteurianum DSM 525 EET within MFCDSM.
Keywords
Clostridium pasteurianum DSM 525; glycerol; mediated electron transfer (MET); neutral red (NR); methyl viologen (MV)
Subject
Chemistry and Materials Science, Electrochemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
