Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend

Nicholas L.F. Gallina; Nicole I Tardi; Xilin Li; Alvin Cai; Mandy Horn; Bruce Applegate; Lavanya Reddivari; Arun K. Bhunia

doi:10.20944/preprints202409.0922.v1

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preprints.org > biology and life sciences > immunology and microbiology > doi: 10.20944/preprints202409.0922.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend

Nicholas L.F. Gallina

^* ,

Version 1 : Received: 10 September 2024 / Approved: 11 September 2024 / Online: 12 September 2024 (11:26:45 CEST)

How to cite: Gallina, N. L.; Tardi, N. I.; Li, X.; Cai, A.; Horn, M.; Applegate, B.; Reddivari, L.; Bhunia, A. K. Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend. Preprints 2024, 2024090922. https://doi.org/10.20944/preprints202409.0922.v1 Gallina, N. L.; Tardi, N. I.; Li, X.; Cai, A.; Horn, M.; Applegate, B.; Reddivari, L.; Bhunia, A. K. Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend. Preprints 2024, 2024090922. https://doi.org/10.20944/preprints202409.0922.v1

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MDPI and ACS Style

Gallina, N. L.; Tardi, N. I.; Li, X.; Cai, A.; Horn, M.; Applegate, B.; Reddivari, L.; Bhunia, A. K. Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend. Preprints 2024, 2024090922. https://doi.org/10.20944/preprints202409.0922.v1

APA Style

Gallina, N. L., Tardi, N. I., Li, X., Cai, A., Horn, M., Applegate, B., Reddivari, L., & Bhunia, A. K. (2024). Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend. Preprints. https://doi.org/10.20944/preprints202409.0922.v1

Chicago/Turabian Style

Gallina, N. L., Lavanya Reddivari and Arun K. Bhunia. 2024 "Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend" Preprints. https://doi.org/10.20944/preprints202409.0922.v1

Abstract

Gut dysbiosis and an inflamed bowel are growing concerns in mammals, including dogs. Probiotic supplements have been used to restore the natural microbial community and improve gastrointestinal health. Biofilm formation, antimicrobial activities, and immunological responses of probiotics are crucial to improving gut health. Thus, we tested a commercial probiotic blend (LabMAX-3), a canine kibble additive comprising Lactobacillus acidophilus, Lacticasibacillus casei, and Enterococcus faecium for their ability to inactivate common enteric pathogens; their ability to form biofilms, epithelial cell adhesion and anti-inflammatory response on Madin-Darby Canine Kidney (MDCK) cell line. Probiotic LabMAX-3 blend, or individual isolates, showed a strong inhibitory effect against Salmonella enterica, Listeria monocytogenes, enterotoxigenic Escherichia coli, and Campylobacter jejuni. LabMAX-3 formed biofilms comparable to Staphylococcus aureus. LabMAX-3 adhesion to the MDCK cell line (with or without lipopolysaccharide, LPS) pretreatment showed comparable adhesion and biofilm formation (p < 0.05) to L. casei ATCC 334 used as a control. LabMAX-3 had no cytotoxic effects on the MDCK cell line during 1 h exposure. The IL-10 and TNFα ratio of LabMAX-3, compared to the L. casei control, showed a significant increase (p < 0.05), indicating a more pronounced anti-inflammatory response. The data show that LabMAX-3, a canine kibble supplement, can improve gastrointestinal health.

Keywords

Probiotics; biofilm; antimicrobial activity; adhesion; pathogens; canine gut health; anti-inflammatory response

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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