Aswad, M.; Pechkovsky, A.; Ghanayiem, N.; Dotan, Y.; Louria-Hayon, I. CBD-X Extracts in Asthma Management: Reducing Th2-Driven Cytokine Secretion and Neutrophil/Eosonophil Activity. Preprints2024, 2024090963. https://doi.org/10.20944/preprints202409.0963.v1
APA Style
Aswad, M., Pechkovsky, A., Ghanayiem, N., Dotan, Y., & Louria-Hayon, I. (2024). CBD-X Extracts in Asthma Management: Reducing Th2-Driven Cytokine Secretion and Neutrophil/Eosonophil Activity. Preprints. https://doi.org/10.20944/preprints202409.0963.v1
Chicago/Turabian Style
Aswad, M., Yaniv Dotan and Igal Louria-Hayon. 2024 "CBD-X Extracts in Asthma Management: Reducing Th2-Driven Cytokine Secretion and Neutrophil/Eosonophil Activity" Preprints. https://doi.org/10.20944/preprints202409.0963.v1
Abstract
Background/Objectives: Asthma is a chronic inflammatory disorder of the airways affecting over 10% of the global population. It is characterized by airway inflammation, mucus hypersecretion, and bronchial hyperresponsiveness, driven predominantly by type 2 helper T cells (Th2) and type 2 innate lymphoid cells (ILC2s) in a subset of patients. However, a significant portion of asthmatic individuals present with "type 2-low" asthma, often refractory to standard inhaled corticosteroid (ICS) therapy. Therefore, developing innovative therapeutic strategies has become essential. Recent studies have highlighted cannabidiol (CBD) as a promising anti-inflammatory agent capable of modulating immune responses. This study investigates the therapeutic potential of a high-CBD extract (CBD-X) in asthma; Methods: We evaluated the effects of CBD-X on cells involved in asthma pathogenesis using primary human Th2 cells, neutrophils and asthma mouse model; Results: Our findings indicate that CBD-X extract inhibits Th2 differentiation and reduces the secretion of IL-5 and IL-13, which are crucial cytokines in asthma. Additionally, CBD-X significantly reduces pro-inflammatory cytokines IL-8 and IL-6 in neutrophils and impairs their migration, a critical step in airway inflammation. In a murine asthma model, CBD-X administration led to marked downregulation of IgE and pro-asthmatic cytokines, along with reduced leukocyte, eosinophil, and neutrophil infiltration in lung tissues; Conclusions: These results suggest that CBD-X extract could offer a novel, complementary approach to managing both type 2-high and type 2-low asthma by targeting key inflammatory pathways and modulating immune cell behavior.
Keywords
CBD extracts; asthma; type 2 helper T cells (Th2); neutrophil; Eosonophil; OVA-Induced Asthmatic mouse model; cytokines; migration; IgE
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
