Version 1
: Received: 12 September 2024 / Approved: 12 September 2024 / Online: 13 September 2024 (11:32:13 CEST)
How to cite:
Freitas, E. C. V. D.; Santos, F. A. D.; Lopes, M. R. V.; Sousa Júnior, D. L. D.; Yafawi, T. T. A.; Araújo, A. C. F.; Freitas, P. R.; Menezes, I. R. A. D.; Coutinho, H. D. M.; Leandro, M. K. D. N. S. Co-extraction of DNA and RNA from Candida albicans Using a Chemical Method in Conjunction with Silicon Carbide with Few Cells. Preprints2024, 2024091037. https://doi.org/10.20944/preprints202409.1037.v1
Freitas, E. C. V. D.; Santos, F. A. D.; Lopes, M. R. V.; Sousa Júnior, D. L. D.; Yafawi, T. T. A.; Araújo, A. C. F.; Freitas, P. R.; Menezes, I. R. A. D.; Coutinho, H. D. M.; Leandro, M. K. D. N. S. Co-extraction of DNA and RNA from Candida albicans Using a Chemical Method in Conjunction with Silicon Carbide with Few Cells. Preprints 2024, 2024091037. https://doi.org/10.20944/preprints202409.1037.v1
Freitas, E. C. V. D.; Santos, F. A. D.; Lopes, M. R. V.; Sousa Júnior, D. L. D.; Yafawi, T. T. A.; Araújo, A. C. F.; Freitas, P. R.; Menezes, I. R. A. D.; Coutinho, H. D. M.; Leandro, M. K. D. N. S. Co-extraction of DNA and RNA from Candida albicans Using a Chemical Method in Conjunction with Silicon Carbide with Few Cells. Preprints2024, 2024091037. https://doi.org/10.20944/preprints202409.1037.v1
APA Style
Freitas, E. C. V. D., Santos, F. A. D., Lopes, M. R. V., Sousa Júnior, D. L. D., Yafawi, T. T. A., Araújo, A. C. F., Freitas, P. R., Menezes, I. R. A. D., Coutinho, H. D. M., & Leandro, M. K. D. N. S. (2024). Co-extraction of DNA and RNA from Candida albicans Using a Chemical Method in Conjunction with Silicon Carbide with Few Cells. Preprints. https://doi.org/10.20944/preprints202409.1037.v1
Chicago/Turabian Style
Freitas, E. C. V. D., Henrique Douglas Melo Coutinho and Maria Karollyna do Nascimento Silva Leandro. 2024 "Co-extraction of DNA and RNA from Candida albicans Using a Chemical Method in Conjunction with Silicon Carbide with Few Cells" Preprints. https://doi.org/10.20944/preprints202409.1037.v1
Abstract
The study aimed to optimize protocols for the joint extraction of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from 0.025 x 106 CFU of Candida albicans, aiming to overcome challenges in the extraction of these genetic materials. From this, treated Silicon Carbide (SiC) granules were added to fungal samples from methods 1, 2 and 3 obtained from aliquots of BHI or Sabouraud medium to cause cell lysis and enable the isolation of these macromolecules by phenol and chloroform. The concentration and integrity of nucleic acids were obtained, respectively, by spectrophotometry using A260/A280 ratios and 1% agarose gel electrophoresis. Therefore, method 3 is the one that most comprises samples considered pure of both DNA and RNA, simultaneously. Furthermore, the presence of intact RNAs corresponding to the base pair size, such as 5.8 S rRNA and tRNA, was verified during electrophoresis, taking into account the particularities of RNA, which makes it very unstable and easily degraded. Therefore, it results in a faster and simpler method in addition to obtaining promising results using minimal amounts of biological sample, thus offering a valuable alternative for small laboratories working with molecular biology.
Keywords
Chloriform-phenol; Extraction; Nucleic acids
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
