Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights

Talita Émile Ribeiro Adelino; Sílvia Helena Sousa Pietra Pedroso; Maurício Lima; Luiz Marcelo Ribeiro Tomé; Natália Rocha Guimarães; Vagner Fonseca; Paulo Eduardo de Souza da Silva; Keldenn Melo Farias Moreno; Ana Cândida Araújo e Silva; Náthale Rodrigues Pinheiro; Carolina Senra Alves de Souza; Luiz Carlos Junior Alcantara; Marta Giovanetti; Felipe Campos de Melo Iani

doi:10.20944/preprints202409.1137.v1

How to cite: Adelino, T. É. R.; Pedroso, S. H. S. P.; Lima, M.; Tomé, L. M. R.; Guimarães, N. R.; Fonseca, V.; Silva, P. E. D. S. D.; Moreno, K. M. F.; Silva, A. C. A. E.; Pinheiro, N. R.; Souza, C. S. A. D.; Alcantara, L. C. J.; Giovanetti, M.; Iani, F. C. D. M. Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights. Preprints 2024, 2024091137. https://doi.org/10.20944/preprints202409.1137.v1 Adelino, T. É. R.; Pedroso, S. H. S. P.; Lima, M.; Tomé, L. M. R.; Guimarães, N. R.; Fonseca, V.; Silva, P. E. D. S. D.; Moreno, K. M. F.; Silva, A. C. A. E.; Pinheiro, N. R.; Souza, C. S. A. D.; Alcantara, L. C. J.; Giovanetti, M.; Iani, F. C. D. M. Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights. Preprints 2024, 2024091137. https://doi.org/10.20944/preprints202409.1137.v1

Abstract

This study examines a case involving a 7-year-old child who developed dengue symptoms following Qdenga vaccination. Despite initial negative diagnostic results, molecular analysis confirmed an infection with DENV4. Next-generation sequencing detected viral RNA from both DENV2 and DENV4 serotypes, which were identified as vaccine-derived strains using specific primers. Phylogenetic analysis further confirmed that these sequences belonged to the Qdenga vaccine rather than circulating wild-type viruses. This case underscores the critical need for precise diagnostic interpretation in vaccinated individuals to avoid misdiagnosis and to strengthen public health surveillance. A comprehensive understanding of vaccine-induced viremia is essential for refining dengue surveillance, improving diagnostic accuracy, and informing public health strategies in endemic regions.

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Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights

Abstract

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