Preprint Article Version 1 This version is not peer-reviewed

Whole-Genome Sequencing Analysis of Antimicrobial Resistance, Virulence Factors, and Genetic Diversity of Salmonella Isolated from Wenzhou, China

Yafang Jin
,
Yi Li
,
Shaojie Huang
,
Chengji Hong
,
Xucong Feng
,
Huidi Cai
,
Yanmei Xia
,
Shengkai Li
,
Leyi Zhang
,
Yongliang Lou
* ,
Wanchun Guan
*
Version 1 : Received: 14 September 2024 / Approved: 15 September 2024 / Online: 16 September 2024 (03:20:34 CEST)

How to cite: Jin, Y.; Li, Y.; Huang, S.; Hong, C.; Feng, X.; Cai, H.; Xia, Y.; Li, S.; Zhang, L.; Lou, Y.; Guan, W. Whole-Genome Sequencing Analysis of Antimicrobial Resistance, Virulence Factors, and Genetic Diversity of Salmonella Isolated from Wenzhou, China. Preprints 2024, 2024091151. https://doi.org/10.20944/preprints202409.1151.v1 Jin, Y.; Li, Y.; Huang, S.; Hong, C.; Feng, X.; Cai, H.; Xia, Y.; Li, S.; Zhang, L.; Lou, Y.; Guan, W. Whole-Genome Sequencing Analysis of Antimicrobial Resistance, Virulence Factors, and Genetic Diversity of Salmonella Isolated from Wenzhou, China. Preprints 2024, 2024091151. https://doi.org/10.20944/preprints202409.1151.v1

Abstract

Salmonella species are important foodborne pathogens worldwide. Salmonella pathogenicity is associated with multiple virulence factors and enhanced antimicrobial resistance. To determine the molecular characteristics and genetic correlations of Salmonella, 24 strains of Salmonella isolated from different sources (raw poultry, human stool, and food) in the Wenzhou area were investigated to determine the distribution of antimicrobial resistance and virulence determinants using whole-genome sequencing (WGS). Aminoglycoside-resistance genes were detected in all samples. Over half of the samples found antimicrobial resistance genes (ARGs) and point mutations for several clinically frequently used antibiotic, β-lactamases, tetracyclines, and fluoroquinolones. Of these strains, 62.5% were predicted to be multidrug-resistant (MDR). The quinolone-modifying enzyme gene aac(6')-Ib-cr, detected in five samples (S1–S4 and S10), were located on integrons. Analysis of Salmonella pathogenicity island (SPI) profiles suggests that serotypes with close genetic relationships share the same distribution of virulence factors, revealing a link between genotype and SPI profiles. CgMLST analysis indicated that five isolates S14–S18 were closely related to strains originating from the UK, suggesting that they may have been introduced from the UK. Data from this study may enrich the molecular traceability database for Salmonella and provide a basis for effective public health policies.

Keywords

Salmonella; antimicrobial resistance; pathogenicity islands; cgMLST; Whole genome sequencing

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.