PreprintArticleVersion 1This version is not peer-reviewed
Amino Acid-based Protein-mimic Hydrogel Incorporating Pro-regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds
Lu, Y.; Su, S.; Chu, C.-C.; Kobayashi, Y.; Masoud, A.-R.; Peng, H.; Lien, N.; He, M.; Vuong, C.; Tran, R.; Hong, S. Amino Acid-based Protein-mimic Hydrogel Incorporating Pro-regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds. Preprints2024, 2024091242. https://doi.org/10.20944/preprints202409.1242.v1
APA Style
Lu, Y., Su, S., Chu, C. C., Kobayashi, Y., Masoud, A. R., Peng, H., Lien, N., He, M., Vuong, C., Tran, R., & Hong, S. (2024). Amino Acid-based Protein-mimic Hydrogel Incorporating Pro-regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds. Preprints. https://doi.org/10.20944/preprints202409.1242.v1
Chicago/Turabian Style
Lu, Y., Ryan Tran and Song Hong. 2024 "Amino Acid-based Protein-mimic Hydrogel Incorporating Pro-regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds" Preprints. https://doi.org/10.20944/preprints202409.1242.v1
Abstract
Pro-regenerative lipid mediator 1 (PreM1) is a specialized pro-resolving lipid mediator capable of promoting wound healing and regenerative functions of mesenchymal stem cells (MSCs), en-dothelial cells, and macrophages. The third-degree (3o) burn healing and regenerative functions of MSCs are also enhanced by ACgel1, an arginine and chitosan-based protein-mimic hybrid hy-drogel. Adipose-tissue derived microvascular fragments (MVFs) are native vascularization units and a rich source of MSCs, endothelial cells, and perivascular cells for tissue regeneration. Here we describe an innovative PreM1-MVFs-ACgel1 construct that incorporates PreM1 and MVFs into ACgel1 via optimal design and fabrication is capable of delivering PreM1 to 3o burn wounds at least up to 7 days post-burn (dpb), and of scaffolding and delivering MVFs, which promoted 3o burn the healing in mice, including vascularization and collagen formation. The re-epithelization and closure of 3o burn wounds were promoted by ACgel1, MVFs, PreM1, MVFs-ACgel1, PreM1-ACgel1, or PreM1-MVFs-ACgel1 at certain time-point(s) while PreM1-MVFs-ACgel1 was most effective. The PreM1-ACgel1 and MVFs-ACgel1 also promoted blood vessel regeneration of the 3o burns. These PreM1- and/or MVFs- functionalized ACgel1 have nonexistent or minimal graft-donor requirements and are promising adjuvant therapies for overcoming the drawbacks of current skin grafting or substituting for treating severe deep burns.
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
