PreprintArticleVersion 1This version is not peer-reviewed
The Lower Pre-Transplant Hepatitis C Antibody in Il-28B RS8099917 Tt Genotype with Higher miRNA-122 Expression May Correlate to the Acute Cellular Rejection After Ldlt
Version 1
: Received: 16 September 2024 / Approved: 16 September 2024 / Online: 17 September 2024 (12:15:40 CEST)
How to cite:
Chiu, K.-W.; Lin, Y. C.; Li, W. F.; Huang, K. T.; Hsu, L.-W.; Wang, C.-C. The Lower Pre-Transplant Hepatitis C Antibody in Il-28B RS8099917 Tt Genotype with Higher miRNA-122 Expression May Correlate to the Acute Cellular Rejection After Ldlt. Preprints2024, 2024091303. https://doi.org/10.20944/preprints202409.1303.v1
Chiu, K.-W.; Lin, Y. C.; Li, W. F.; Huang, K. T.; Hsu, L.-W.; Wang, C.-C. The Lower Pre-Transplant Hepatitis C Antibody in Il-28B RS8099917 Tt Genotype with Higher miRNA-122 Expression May Correlate to the Acute Cellular Rejection After Ldlt. Preprints 2024, 2024091303. https://doi.org/10.20944/preprints202409.1303.v1
Chiu, K.-W.; Lin, Y. C.; Li, W. F.; Huang, K. T.; Hsu, L.-W.; Wang, C.-C. The Lower Pre-Transplant Hepatitis C Antibody in Il-28B RS8099917 Tt Genotype with Higher miRNA-122 Expression May Correlate to the Acute Cellular Rejection After Ldlt. Preprints2024, 2024091303. https://doi.org/10.20944/preprints202409.1303.v1
APA Style
Chiu, K. W., Lin, Y. C., Li, W. F., Huang, K. T., Hsu, L. W., & Wang, C. C. (2024). The Lower Pre-Transplant Hepatitis C Antibody in Il-28B RS8099917 Tt Genotype with Higher miRNA-122 Expression May Correlate to the Acute Cellular Rejection After Ldlt. Preprints. https://doi.org/10.20944/preprints202409.1303.v1
Chicago/Turabian Style
Chiu, K., Li-Wen Hsu and Chih-Chi Wang. 2024 "The Lower Pre-Transplant Hepatitis C Antibody in Il-28B RS8099917 Tt Genotype with Higher miRNA-122 Expression May Correlate to the Acute Cellular Rejection After Ldlt" Preprints. https://doi.org/10.20944/preprints202409.1303.v1
Abstract
Background: miRNA-122 has been reported to be a useful tool for monitoring ACR after LT. IL-28B SNP rs8099917 genotype is known to contribute to the control of HCV infection. We sought to investigate the relationship between IL-28B SNP rs8099917 genotype and miRNA-122 expression and immune mechanism of ACR after LT using hepatitis C antibody calibration. Patients and Methods: A total of 45 patients with chronic hepatitis C received LDLT. IL-28B SNP rs8099917 genotyping was used to divide TT and GT groups. The relative expression levels of miRNA-122 were calculated by quantitative PCR as 2[(Ct of miR-122) (Ct of U6)] normalized to a reference gene and compared to a control sample, and the anti-HCV titers before and after LT were tracked to observe the relationship with ACR. Results: In the genotype of rs8099917, TT was significantly more associated with higher serum miRNA-122 levels than GT (p = 0.029). The ACR was significantly higher in genotype TT than in GT (p = 0.002). TT was significantly associated with low levels of pre-LT anti-HCV compared with GT (p = 0.022). Multivariate analysis with 95% confidence intervals showed a significant association between rs8099917 genotype TT, lower pre-LT and higher post-LT anti-HCV and ACR. Conclusion: Based on our current data, IL-28B SNP rs8099917 genotype TT may express higher miRNA-122 and correlate with lower Pre-LT and higher Post-LT anti-HCV titers, and may correlate to the pathogenesis of ACR after LT.
Keywords
acute cellular rejection; hepatitis C virus; IL-28B, liver transplantation; microRNA-122; single nucleotide polymorphism
Subject
Medicine and Pharmacology, Transplantation
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
