Version 1
: Received: 23 September 2024 / Approved: 24 September 2024 / Online: 24 September 2024 (05:01:42 CEST)
How to cite:
Bojko, M.; Kędra, M.; Adamska, A.; Jakubowska, Z.; Tuleja, M.; Mysliwa-Kurdziel, B. Induction and Characteristics of Medicinal Plant Tussilago farfara L. in Callus Culture. Preprints2024, 2024091850. https://doi.org/10.20944/preprints202409.1850.v1
Bojko, M.; Kędra, M.; Adamska, A.; Jakubowska, Z.; Tuleja, M.; Mysliwa-Kurdziel, B. Induction and Characteristics of Medicinal Plant Tussilago farfara L. in Callus Culture. Preprints 2024, 2024091850. https://doi.org/10.20944/preprints202409.1850.v1
Bojko, M.; Kędra, M.; Adamska, A.; Jakubowska, Z.; Tuleja, M.; Mysliwa-Kurdziel, B. Induction and Characteristics of Medicinal Plant Tussilago farfara L. in Callus Culture. Preprints2024, 2024091850. https://doi.org/10.20944/preprints202409.1850.v1
APA Style
Bojko, M., Kędra, M., Adamska, A., Jakubowska, Z., Tuleja, M., & Mysliwa-Kurdziel, B. (2024). Induction and Characteristics of Medicinal Plant <em>Tussilago farfara L.</em> in Callus Culture. Preprints. https://doi.org/10.20944/preprints202409.1850.v1
Chicago/Turabian Style
Bojko, M., Monika Tuleja and Beata Mysliwa-Kurdziel. 2024 "Induction and Characteristics of Medicinal Plant <em>Tussilago farfara L.</em> in Callus Culture" Preprints. https://doi.org/10.20944/preprints202409.1850.v1
Abstract
Tussilago farfara L. is a traditional medicinal plant valued for its potentially health-promoting metabolites. Its herbal raw material has been recognized and used since ancient times and continues to be widely used in traditional medicine. Introducing these plants to in vitro cultivation is a challenging task, but once the protocol is developed, such cultures can provide an abundant and inexhaustible source of plant material. In this study, we report the successful induction and growth of vigorous T. farfara L. callus in vitro. Callus induction was achieved on solid media in darkness, whereas it appeared inefficient under light conditions and in suspension culture. We present a detailed description of callus growth kinetics, morphological analysis, photosynthetic activity and biochemical parameters (including protein content and photosynthetic pigments) supported by histological studies. Furthermore, we observed potential for organogenesis and somatic embryogenesis. This method for in vitro propagation of T. farfara, along with callus culture maintenance, offers a wide range of applications in pharmacy and could benefit the environment by minimizing the depletion of natural populations of this species.
Keywords
callus induction; callus; chlorophyll in vivo fluorescence; coltsfoot; organogenesis; histology; chlorophylls and carotenoids content; callus growth parameters
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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