Genetic Engineering in Bacteria, Fungi, and Oomycetes

Piao Yang; Abraham Condrich; Ling Lu; Sean Scranton; Camina Hebner; Mohsen Sheykhhasan; Muhammad Azam Ali

doi:10.20944/preprints202409.1958.v1

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preprints.org > biology and life sciences > biology and biotechnology > doi: 10.20944/preprints202409.1958.v1

Preprint Review Version 1 This version is not peer-reviewed

Genetic Engineering in Bacteria, Fungi, and Oomycetes

^* ,

Version 1 : Received: 24 September 2024 / Approved: 25 September 2024 / Online: 25 September 2024 (08:56:49 CEST)

How to cite: Yang, P.; Condrich, A.; Lu, L.; Scranton, S.; Hebner, C.; Sheykhhasan, M.; Ali, M. A. Genetic Engineering in Bacteria, Fungi, and Oomycetes. Preprints 2024, 2024091958. https://doi.org/10.20944/preprints202409.1958.v1 Yang, P.; Condrich, A.; Lu, L.; Scranton, S.; Hebner, C.; Sheykhhasan, M.; Ali, M. A. Genetic Engineering in Bacteria, Fungi, and Oomycetes. Preprints 2024, 2024091958. https://doi.org/10.20944/preprints202409.1958.v1

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MDPI and ACS Style

Yang, P.; Condrich, A.; Lu, L.; Scranton, S.; Hebner, C.; Sheykhhasan, M.; Ali, M. A. Genetic Engineering in Bacteria, Fungi, and Oomycetes. Preprints 2024, 2024091958. https://doi.org/10.20944/preprints202409.1958.v1

APA Style

Yang, P., Condrich, A., Lu, L., Scranton, S., Hebner, C., Sheykhhasan, M., & Ali, M. A. (2024). Genetic Engineering in Bacteria, Fungi, and Oomycetes. Preprints. https://doi.org/10.20944/preprints202409.1958.v1

Chicago/Turabian Style

Yang, P., Mohsen Sheykhhasan and Muhammad Azam Ali. 2024 "Genetic Engineering in Bacteria, Fungi, and Oomycetes" Preprints. https://doi.org/10.20944/preprints202409.1958.v1

Abstract

Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances in genetic engineering techniques for bacteria, fungi, and oomycetes, with a focus on CRISPR-Cas systems. In bacteria, CRISPR-Cas9 has enabled precise genome editing, enhancing applications in antibiotic production and metabolic engineering. For fungi, despite challenges associated with their complex cell structures, CRISPR/Cas9 has advanced the production of enzymes and secondary metabolites. In oomycetes, significant plant pathogens, modified Agrobacterium-mediated transformation and CRISPR/Cas12a have contributed to developing disease-resistant crops. The review provides a comparative analysis of genetic engineering efficiencies across these microorganisms and addresses ethical and regulatory considerations. Future research directions include refining genetic tools to improve efficiency and expand applicability in non-model organisms. This comprehensive overview highlights the transformative potential of genetic engineering in microbiology and its implications for addressing global challenges in agriculture, medicine, and biotechnology.

Keywords

Genetic engineering; CRISPR; Bacteria; Fungi; Oomycetes; GEMs

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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