Version 1
: Received: 26 September 2024 / Approved: 27 September 2024 / Online: 29 September 2024 (05:40:16 CEST)
How to cite:
Sosnowska, M.; Pitula, E.; Janik, M.; Bruździak, P.; Śmietana, M.; Olszewski, M.; Nidzworski, D.; Gromadzka, B. Peptide-Based Rapid and Selective Detection of Mercury in Aqueous Samples with Micro-Volume Glass Capillary Fluorometer. Preprints2024, 2024092237. https://doi.org/10.20944/preprints202409.2237.v1
Sosnowska, M.; Pitula, E.; Janik, M.; Bruździak, P.; Śmietana, M.; Olszewski, M.; Nidzworski, D.; Gromadzka, B. Peptide-Based Rapid and Selective Detection of Mercury in Aqueous Samples with Micro-Volume Glass Capillary Fluorometer. Preprints 2024, 2024092237. https://doi.org/10.20944/preprints202409.2237.v1
Sosnowska, M.; Pitula, E.; Janik, M.; Bruździak, P.; Śmietana, M.; Olszewski, M.; Nidzworski, D.; Gromadzka, B. Peptide-Based Rapid and Selective Detection of Mercury in Aqueous Samples with Micro-Volume Glass Capillary Fluorometer. Preprints2024, 2024092237. https://doi.org/10.20944/preprints202409.2237.v1
APA Style
Sosnowska, M., Pitula, E., Janik, M., Bruździak, P., Śmietana, M., Olszewski, M., Nidzworski, D., & Gromadzka, B. (2024). Peptide-Based Rapid and Selective Detection of Mercury in Aqueous Samples with Micro-Volume Glass Capillary Fluorometer. Preprints. https://doi.org/10.20944/preprints202409.2237.v1
Chicago/Turabian Style
Sosnowska, M., Dawid Nidzworski and Beata Gromadzka. 2024 "Peptide-Based Rapid and Selective Detection of Mercury in Aqueous Samples with Micro-Volume Glass Capillary Fluorometer" Preprints. https://doi.org/10.20944/preprints202409.2237.v1
Abstract
Mercury, a toxic heavy metal produced through both natural and anthropogenic processes, is found in all major Earth’s systems. Mercury's bioaccumulation characteristics in the human body have a significant impact on the liver, kidneys, brain, and muscles. In order to detect Hg2+ ions, a highly sensitive and specific fluorescent biosensor has been developed using a novel, modified seven amino acid peptide, FY7. The tyrosine ring in the FY7 peptide sequence forms a 2:1 complex with Hg2+ ions that are present in the water-based sample. As a result, the peptide's fluorescence emission decreases with higher concentrations of Hg2+. The FY7 peptide's performance was tested in the presence of Hg2+ ions and other metal ions, revealing its sensitivity and stability despite high concentrations. Conformational changes to the FY7 structure were confirmed by FTIR studies. Simultaneously, we designed a miniaturized setup to support an in-house-developed micro-volume capillary container for volume fluorometry measurements. We compared and verified the results from the micro-volume system with those from the commercial setup. The micro-volume capillary system accommodated only 2.9 µL of sample volume, allowing for rapid, sensitive, and selective detection of toxic mercury (II) ions ranging from 0.02 µM.
Environmental and Earth Sciences, Water Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
