Version 1
: Received: 1 October 2024 / Approved: 1 October 2024 / Online: 1 October 2024 (10:44:43 CEST)
How to cite:
KIM, M.; Lee, H. H.; Won, S. D.; Jang, Y. S.; Kim, B. G.; Cho, N. H.; Choi, Y. D.; Han, H. H.; Chung, J. S. Exploring the Therapeutic Potential of Nicotinamide‐N‐Methyltransferase in Clear Cell Renal Cell Carcinoma. Preprints2024, 2024100041. https://doi.org/10.20944/preprints202410.0041.v1
KIM, M.; Lee, H. H.; Won, S. D.; Jang, Y. S.; Kim, B. G.; Cho, N. H.; Choi, Y. D.; Han, H. H.; Chung, J. S. Exploring the Therapeutic Potential of Nicotinamide‐N‐Methyltransferase in Clear Cell Renal Cell Carcinoma. Preprints 2024, 2024100041. https://doi.org/10.20944/preprints202410.0041.v1
KIM, M.; Lee, H. H.; Won, S. D.; Jang, Y. S.; Kim, B. G.; Cho, N. H.; Choi, Y. D.; Han, H. H.; Chung, J. S. Exploring the Therapeutic Potential of Nicotinamide‐N‐Methyltransferase in Clear Cell Renal Cell Carcinoma. Preprints2024, 2024100041. https://doi.org/10.20944/preprints202410.0041.v1
APA Style
KIM, M., Lee, H. H., Won, S. D., Jang, Y. S., Kim, B. G., Cho, N. H., Choi, Y. D., Han, H. H., & Chung, J. S. (2024). Exploring the Therapeutic Potential of Nicotinamide‐N‐Methyltransferase in Clear Cell Renal Cell Carcinoma. Preprints. https://doi.org/10.20944/preprints202410.0041.v1
Chicago/Turabian Style
KIM, M., Hyun Ho Han and Jin Soo Chung. 2024 "Exploring the Therapeutic Potential of Nicotinamide‐N‐Methyltransferase in Clear Cell Renal Cell Carcinoma" Preprints. https://doi.org/10.20944/preprints202410.0041.v1
Abstract
Clear cell renal cell carcinoma (ccRCC) is a highly aggressive subtype of renal cancer, often associated with poor prognosis and high mortality rates. In this study, we identified Nicotinamide N-methyltransferase (NNMT) as a key gene involved in the pathogenesis of ccRCC through RNA sequencing analysis of tumor and normal tissues from 14 patients. NNMT was among the top five upregulated genes, and its high expression was significantly correlated with reduced overall, progression-free, and disease-specific survival in patients, as well as advanced pathological stages and higher histologic grades. Gene ontology (GO) enrichment analysis revealed NNMT's role in biological processes such as signal transduction and immune response. Immune cell infiltration analysis indicated that high NNMT expression was associated with increased infiltration of activated NK cells and macrophages, while low expression correlated with a higher presence of regulatory T cells. Functional studies using siRNA-mediated NNMT knockdown in ccRCC cell lines demonstrated a significant reduction in cell proliferation, with further analysis revealing altered gene expression profiles related to cell adhesion and calcium ion binding. These findings suggest that NNMT not only plays a pivotal role in ccRCC aggressiveness but also may serve as a valuable prognostic biomarker and a potential therapeutic target for improving clinical outcomes in patients with this malignancy.
Keywords
NNMT; ccRCC; biomarker
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.