Version 1
: Received: 30 September 2024 / Approved: 1 October 2024 / Online: 3 October 2024 (08:00:07 CEST)
How to cite:
Frankowska, N.; Szarek, K.; Iwanicki, A.; Wultańska, D.; Pituch, H.; Kabała, M.; Negri, A.; Obuchowski, M.; Hinc, K. The phi027 Bacteriophage Influences Physiology and Virulence of the Lysogenic Strain of Clostridioides difficile. Preprints2024, 2024100063. https://doi.org/10.20944/preprints202410.0063.v1
Frankowska, N.; Szarek, K.; Iwanicki, A.; Wultańska, D.; Pituch, H.; Kabała, M.; Negri, A.; Obuchowski, M.; Hinc, K. The phi027 Bacteriophage Influences Physiology and Virulence of the Lysogenic Strain of Clostridioides difficile. Preprints 2024, 2024100063. https://doi.org/10.20944/preprints202410.0063.v1
Frankowska, N.; Szarek, K.; Iwanicki, A.; Wultańska, D.; Pituch, H.; Kabała, M.; Negri, A.; Obuchowski, M.; Hinc, K. The phi027 Bacteriophage Influences Physiology and Virulence of the Lysogenic Strain of Clostridioides difficile. Preprints2024, 2024100063. https://doi.org/10.20944/preprints202410.0063.v1
APA Style
Frankowska, N., Szarek, K., Iwanicki, A., Wultańska, D., Pituch, H., Kabała, M., Negri, A., Obuchowski, M., & Hinc, K. (2024). The phi027 Bacteriophage Influences Physiology and Virulence of the Lysogenic Strain of <em>Clostridioides difficile</em>. Preprints. https://doi.org/10.20944/preprints202410.0063.v1
Chicago/Turabian Style
Frankowska, N., Michał Obuchowski and Krzysztof Hinc. 2024 "The phi027 Bacteriophage Influences Physiology and Virulence of the Lysogenic Strain of <em>Clostridioides difficile</em>" Preprints. https://doi.org/10.20944/preprints202410.0063.v1
Abstract
Clostridioides difficile, the causative agent of C. difficile infections (CDI), can be naturally infected by bacterial viruses known as bacteriophages. All characterized bacteriophages of this bacterium are temperate, meaning that upon infection their genetic material integrates and replicates with host’s genome. Such lysogenic strains can exhibit altered physiology and virulence, which in turn can be an important factor for epidemiology of CDI. In this study we characterized the phiCDKH02 bacteriophage infecting clinical isolates of C. difficile belonging to hypervirulent ribotypes 027 and 176. The bacteriophage was found to be identical to phi027. To get some insight into the role of this bacteriophage in physiology of its host and interaction with human colon cells, we made use of CRISPR-Cpf1 technology to cure the lysogenic C. difficile of the prophage. The prophage-free strain exhibited altered sporulation efficiency, lowered adhesion and decreased cytopathic effects to-wards human colon cells. These results emphasize importance of prophages in shaping virulence of C. difficile.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
