Version 1
: Received: 4 October 2024 / Approved: 5 October 2024 / Online: 7 October 2024 (09:52:00 CEST)
How to cite:
Trozzo, S.; Neupane, B.; Foster, P. J. A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging. Preprints2024, 2024100381. https://doi.org/10.20944/preprints202410.0381.v1
Trozzo, S.; Neupane, B.; Foster, P. J. A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging. Preprints 2024, 2024100381. https://doi.org/10.20944/preprints202410.0381.v1
Trozzo, S.; Neupane, B.; Foster, P. J. A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging. Preprints2024, 2024100381. https://doi.org/10.20944/preprints202410.0381.v1
APA Style
Trozzo, S., Neupane, B., & Foster, P. J. (2024). A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging. Preprints. https://doi.org/10.20944/preprints202410.0381.v1
Chicago/Turabian Style
Trozzo, S., Bijita Neupane and Paula J. Foster. 2024 "A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging" Preprints. https://doi.org/10.20944/preprints202410.0381.v1
Abstract
Preclinical cell tracking is enhanced with a multimodal imaging approach. Bioluminescence imaging (BLI) is a highly sensitive optical modality which relies on engineering cells to constitutively express a luciferase gene. Magnetic particle imaging (MPI) is a newer imaging modality which directly detects superparamagnetic iron oxide (SPIO) particles used to label cells. Here, we compare BLI and MPI for imaging cells in vitro and in vivo. Mouse 4T1 breast carcinoma cells were transduced to express firefly luciferase, labeled with SPIO (ProMag) and imaged as cell samples and after subcutaneous injection into mice. For cell samples, the BLI and MPI signals were strongly correlated with cell number. Both modalities presented limitations for imaging cells in vivo. For BLI, weak signal penetration, signal attenuation and scattering prevented detection of cells for mice with hair and for cells far from the tissue surface. For MPI, background signals obscured the detection of low cell numbers due to the limited dynamic range and cell number could not be accurately quantified from in vivo images. It is important to understand the shortcomings of these imaging modalities to develop strategies to improve cellular detection sensitivity.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
