Version 1
: Received: 7 October 2024 / Approved: 7 October 2024 / Online: 8 October 2024 (08:54:06 CEST)
How to cite:
Ishikawa, K.; Suzuki, H.; Tanaka, T.; Kaneko, M. K.; Kato, Y. An Anti-Mouse CXCR5 Monoclonal Antibody (Cx5Mab-3) Shows High Affinity for Flow Cytometry. Preprints2024, 2024100497. https://doi.org/10.20944/preprints202410.0497.v1
Ishikawa, K.; Suzuki, H.; Tanaka, T.; Kaneko, M. K.; Kato, Y. An Anti-Mouse CXCR5 Monoclonal Antibody (Cx5Mab-3) Shows High Affinity for Flow Cytometry. Preprints 2024, 2024100497. https://doi.org/10.20944/preprints202410.0497.v1
Ishikawa, K.; Suzuki, H.; Tanaka, T.; Kaneko, M. K.; Kato, Y. An Anti-Mouse CXCR5 Monoclonal Antibody (Cx5Mab-3) Shows High Affinity for Flow Cytometry. Preprints2024, 2024100497. https://doi.org/10.20944/preprints202410.0497.v1
APA Style
Ishikawa, K., Suzuki, H., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). An Anti-Mouse CXCR5 Monoclonal Antibody (Cx<sub>5</sub>Mab-3) Shows High Affinity for Flow Cytometry. Preprints. https://doi.org/10.20944/preprints202410.0497.v1
Chicago/Turabian Style
Ishikawa, K., Mika K. Kaneko and Yukinari Kato. 2024 "An Anti-Mouse CXCR5 Monoclonal Antibody (Cx<sub>5</sub>Mab-3) Shows High Affinity for Flow Cytometry" Preprints. https://doi.org/10.20944/preprints202410.0497.v1
Abstract
The CXC chemokine receptor 5 (CXCR5) is a member of the G protein-coupled receptor family that is highly expressed in B cells and a subset of T cells such as T follicular helper cells. Various types of cancers, including non-small cell lung cancer, breast cancer, and prostate cancer also express CXCR5; therefore, antibodies that specifically bind to CXCR5 could be useful for clarification of the mechanisms of cancer progression. In this study, we aimed to develop high-affinity monoclonal antibodies (mAbs) for flow cytometry against mouse CXCR5 (mCXCR5). The established anti-mCXCR5 mAb, Cx5Mab-3 (rat IgG2b, kappa), reacted with mCXCR5-overexpressed Chinese hamster ovary (CHO)-K1 (CHO/mCXCR5) by flow cytometry. Kinetic analyses using flow cytometry indicated that the dissociation constants (KD) of Cx5Mab-3 for CHO/mCXCR5 is 7.2 × 10−10 M, indicating that Cx5Mab-3 is useful for detecting mCXCR5 by flow cytometry with high affinity.
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
