Version 1
: Received: 7 October 2024 / Approved: 8 October 2024 / Online: 8 October 2024 (10:24:25 CEST)
How to cite:
Gerhards, N. M.; Vrieling, M.; Dresken, R.; Nguyen-Van Oort, S.; Bordes, L.; Wells, J. M.; de Swart, R. L. Porcine Airway Organoid-Derived Well-Differentiated Epithelial Cultures as a Tool for Characterization of Swine Influenza a Virus Strains. Preprints2024, 2024100552. https://doi.org/10.20944/preprints202410.0552.v1
Gerhards, N. M.; Vrieling, M.; Dresken, R.; Nguyen-Van Oort, S.; Bordes, L.; Wells, J. M.; de Swart, R. L. Porcine Airway Organoid-Derived Well-Differentiated Epithelial Cultures as a Tool for Characterization of Swine Influenza a Virus Strains. Preprints 2024, 2024100552. https://doi.org/10.20944/preprints202410.0552.v1
Gerhards, N. M.; Vrieling, M.; Dresken, R.; Nguyen-Van Oort, S.; Bordes, L.; Wells, J. M.; de Swart, R. L. Porcine Airway Organoid-Derived Well-Differentiated Epithelial Cultures as a Tool for Characterization of Swine Influenza a Virus Strains. Preprints2024, 2024100552. https://doi.org/10.20944/preprints202410.0552.v1
APA Style
Gerhards, N. M., Vrieling, M., Dresken, R., Nguyen-Van Oort, S., Bordes, L., Wells, J. M., & de Swart, R. L. (2024). Porcine Airway Organoid-Derived Well-Differentiated Epithelial Cultures as a Tool for Characterization of Swine Influenza a Virus Strains. Preprints. https://doi.org/10.20944/preprints202410.0552.v1
Chicago/Turabian Style
Gerhards, N. M., Jerry M. Wells and Rik L. de Swart. 2024 "Porcine Airway Organoid-Derived Well-Differentiated Epithelial Cultures as a Tool for Characterization of Swine Influenza a Virus Strains" Preprints. https://doi.org/10.20944/preprints202410.0552.v1
Abstract
Swine influenza A viruses (IAVsw) are important causes of disease in pigs but also constitute a public health risk. IAVsw strains show remarkable differences in pathogenicity. We aimed to generate airway organoids from the porcine lower respiratory tract and use these to establish well-differentiated airway epithelial cell (WD-AEC) cultures grown at air-liquid interface (ALI) for in vitro screening of IAVsw strain virulence. Epithelial cells were isolated from bronchus tissue of juvenile pigs, and airway organoids were cultured in extracellular matrix in medium with human growth factors. Single cell suspensions of these 3D organoids were seeded on transwell filters and differentiated at ALI to form a pseudostratified epithelium containing ciliated cells, mucus-producing cells and tight junctions. Inoculation with a low dose of IAVsw in a low volume inoculum resulted in virus replication without requiring addition of trypsin, and was quantified by detection of viral genome loads in apical washes. Interestingly, inoculation of an H3N2 strain known to cause severe disease in pigs induced greater reduction in trans-epithelial resistance and more damage to tight junctions than H1N2 or H1N1 strains associated with mild disease in pigs. We conclude that the porcine WD-AEC model is useful to assess the virulence of IAVsw strains.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
