Version 1
: Received: 8 October 2024 / Approved: 9 October 2024 / Online: 9 October 2024 (12:21:00 CEST)
How to cite:
Pommiès, L.; Boutal, H.; Fras, D.; Boudergui, K.; Simon, S.; Volland, H. An All-in-One Device for a Rapid and Simple Genetic Detection. Preprints2024, 2024100665. https://doi.org/10.20944/preprints202410.0665.v1
Pommiès, L.; Boutal, H.; Fras, D.; Boudergui, K.; Simon, S.; Volland, H. An All-in-One Device for a Rapid and Simple Genetic Detection. Preprints 2024, 2024100665. https://doi.org/10.20944/preprints202410.0665.v1
Pommiès, L.; Boutal, H.; Fras, D.; Boudergui, K.; Simon, S.; Volland, H. An All-in-One Device for a Rapid and Simple Genetic Detection. Preprints2024, 2024100665. https://doi.org/10.20944/preprints202410.0665.v1
APA Style
Pommiès, L., Boutal, H., Fras, D., Boudergui, K., Simon, S., & Volland, H. (2024). An All-in-One Device for a Rapid and Simple Genetic Detection. Preprints. https://doi.org/10.20944/preprints202410.0665.v1
Chicago/Turabian Style
Pommiès, L., Stéphanie Simon and Hervé Volland. 2024 "An All-in-One Device for a Rapid and Simple Genetic Detection" Preprints. https://doi.org/10.20944/preprints202410.0665.v1
Abstract
Diagnostics often require specialized equipment and trained personnel in laboratory settings, creating a growing need for point-of-care tests (POCT). Among genetic testing methods, Loop-mediated Isothermal Amplification (LAMP) offers a viable solution for developing genetic POCT due to its compatibility with simplified devices. This study aimed to create a genetic test that integrates all steps from sample processing to results while minimizing the complexity, handling, equipment, and time required. Several challenges were addressed to achieve this goal: 1) Developing a buffer for bacterial DNA extraction that is compatible with both LAMP and immunochromatographic tests; 2) Adapting the LAMP protocol for its use with the device; and 3) Optimizing the detection protocol for specific test conditions, with a lateral flow immunoassay format selected for its POCT compatibility. Following these developments, the test was validated using Escherichia coli and non-E. coli strains. A portable heating station was also developed to enable amplification without costly equipment. The resulting genetic POCT achieved 100% sensitivity and 85% specificity, with results available in 60 to 75 minutes. This study demonstrated that our closed-method POCT efficiently performs DNA extraction, amplification, and detection for bacterial identification. The test’s simplicity and cost-effectiveness will support its implementation in various settings.
Keywords
All-in-one device; POCT; Genetic detection
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
