Version 1
: Received: 9 October 2024 / Approved: 9 October 2024 / Online: 10 October 2024 (08:16:00 CEST)
How to cite:
Alanis-Gómez, R. P.; Hernández-Rosas, F.; Olivares-Hernández, J. D.; Rivera-Muñoz, E. M.; Zapatero-Gutiérrez, A.; Méndez-Lozano, N.; Alanis-Gómez, J. R.; Velázquez-Castillo, R. Magnesium Doped Hydroxyapatite Nanofibers for Medicine Applications: Characterization, Antimicrobial Activity, and Cytotoxicity Study. Preprints2024, 2024100734. https://doi.org/10.20944/preprints202410.0734.v1
Alanis-Gómez, R. P.; Hernández-Rosas, F.; Olivares-Hernández, J. D.; Rivera-Muñoz, E. M.; Zapatero-Gutiérrez, A.; Méndez-Lozano, N.; Alanis-Gómez, J. R.; Velázquez-Castillo, R. Magnesium Doped Hydroxyapatite Nanofibers for Medicine Applications: Characterization, Antimicrobial Activity, and Cytotoxicity Study. Preprints 2024, 2024100734. https://doi.org/10.20944/preprints202410.0734.v1
Alanis-Gómez, R. P.; Hernández-Rosas, F.; Olivares-Hernández, J. D.; Rivera-Muñoz, E. M.; Zapatero-Gutiérrez, A.; Méndez-Lozano, N.; Alanis-Gómez, J. R.; Velázquez-Castillo, R. Magnesium Doped Hydroxyapatite Nanofibers for Medicine Applications: Characterization, Antimicrobial Activity, and Cytotoxicity Study. Preprints2024, 2024100734. https://doi.org/10.20944/preprints202410.0734.v1
APA Style
Alanis-Gómez, R. P., Hernández-Rosas, F., Olivares-Hernández, J. D., Rivera-Muñoz, E. M., Zapatero-Gutiérrez, A., Méndez-Lozano, N., Alanis-Gómez, J. R., & Velázquez-Castillo, R. (2024). Magnesium Doped Hydroxyapatite Nanofibers for Medicine Applications: Characterization, Antimicrobial Activity, and Cytotoxicity Study. Preprints. https://doi.org/10.20944/preprints202410.0734.v1
Chicago/Turabian Style
Alanis-Gómez, R. P., José Rafael Alanis-Gómez and Rodrigo Velázquez-Castillo. 2024 "Magnesium Doped Hydroxyapatite Nanofibers for Medicine Applications: Characterization, Antimicrobial Activity, and Cytotoxicity Study" Preprints. https://doi.org/10.20944/preprints202410.0734.v1
Abstract
Magnesium-doped hydroxyapatite (Mg-HAp) nanofibers show promise for medical applications due to their structural similarity to bone mineral and enhanced biological properties, such as improved biocompatibility and antimicrobial activity. This study synthesized Mg-HAp nanofibers using a microwave-assisted hydrothermal method (MAHM) to evaluate their cytotoxicity, biocompatibility, and antimicrobial efficacy compared to commercial hydroxyapatite (HAp). Characterization through X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectroscopy (EDS), and Fourier Transform Infrared Spectroscopy (FTIR) confirmed the successful incorporation of magnesium, producing high-purity, crystalline nanofibers with hexagonal morphology. Rietveld refinement showed slight lattice parameter shortening, indicating Mg2+ ion integration. Cell viability assays (MTT and AlamarBlue) revealed a significant increase in fibroblast proliferation with 2% and 5% Mg-HAp concentrations compared to controls (p<0.05), demonstrating non-cytotoxicity and enhanced biocompatibility. Antimicrobial tests (disk diffusion method, 100 µg/ml) showed that Mg-HAp had strong antibacterial effects against Gram-positive and Gram-negative bacteria, and moderate antifungal activity against Candida albicans. In contrast, commercial HAp showed no antimicrobial effects. These results suggest Mg-HAp nanofibers have significant advantages as biomaterials for medical applications, particularly in preventing implant-related infections, supporting further clinical development.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
