Version 1
: Received: 11 October 2024 / Approved: 12 October 2024 / Online: 12 October 2024 (07:52:27 CEST)
How to cite:
Kirindage, K. G. I. S.; Jayasinghe, A. M. K.; Ko, C.-I.; Ahn, Y.-S.; Heo, S.-J.; Kim, E.-A.; Cho, N.-K.; Ahn, G. Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from Sargassum horneri on UVB-Exposed HaCaT Keratinocytes. Preprints2024, 2024100959. https://doi.org/10.20944/preprints202410.0959.v1
Kirindage, K. G. I. S.; Jayasinghe, A. M. K.; Ko, C.-I.; Ahn, Y.-S.; Heo, S.-J.; Kim, E.-A.; Cho, N.-K.; Ahn, G. Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from Sargassum horneri on UVB-Exposed HaCaT Keratinocytes. Preprints 2024, 2024100959. https://doi.org/10.20944/preprints202410.0959.v1
Kirindage, K. G. I. S.; Jayasinghe, A. M. K.; Ko, C.-I.; Ahn, Y.-S.; Heo, S.-J.; Kim, E.-A.; Cho, N.-K.; Ahn, G. Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from Sargassum horneri on UVB-Exposed HaCaT Keratinocytes. Preprints2024, 2024100959. https://doi.org/10.20944/preprints202410.0959.v1
APA Style
Kirindage, K. G. I. S., Jayasinghe, A. M. K., Ko, C. I., Ahn, Y. S., Heo, S. J., Kim, E. A., Cho, N. K., & Ahn, G. (2024). Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <em>Sargassum horneri</em> on UVB-Exposed HaCaT Keratinocytes. Preprints. https://doi.org/10.20944/preprints202410.0959.v1
Chicago/Turabian Style
Kirindage, K. G. I. S., Nam-Ki Cho and Ginnae Ahn. 2024 "Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <em>Sargassum horneri</em> on UVB-Exposed HaCaT Keratinocytes" Preprints. https://doi.org/10.20944/preprints202410.0959.v1
Abstract
The present study investigated the photoprotective effect of the ultrasonic-assisted ethanol extract (USHE) from Sargassum horneri, a brown seaweed containing fucosterol (6.22 ± 0.06 mg/g) and sulfoquinovosyl glycerolipids (C23H43O11S, C25H45O11S, C25H47O11S, C27H49O11S), against oxidative damage in ultraviolet B (UVB)-exposed HaCaT keratinocytes. USHE indicated the antioxidant activity with increasing ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Moreover, USHE increased cell viability by markedly reducing the production of intracellular reactive oxygen species (ROS) in UVB-exposed HaCaT keratinocytes. Additionally, USHE reduced the apoptosis, and sub-G1 cell population, and increased the mitochondrial membrane potential. Moreover, USHE modulated the protein expression levels of anti-apoptotic molecules (Bcl-xL, Bcl-2, and PARP), and pro-apoptotic molecules (Bax, cleaved caspase-3, p53, cleaved PARP, and cytochrome C). This modulation accorded with the upregulation of cytosolic heme oxygenase (HO)-1, NAD(P)H quinone oxidoreductase 1 (NQO 1), and nuclear factor erythroid-2-related factor 2 (Nrf2), collectively known as components of the antioxidant system. These findings suggest that USHE has a photoprotective effect in UVB-exposed HaCaT keratinocytes and can be utilized to develop cosmeceuticals for UVB protection.
Biology and Life Sciences, Biology and Biotechnology
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