Version 1
: Received: 15 October 2024 / Approved: 15 October 2024 / Online: 16 October 2024 (09:40:53 CEST)
How to cite:
Mirmazloum, I.; Slavov, A. K.; Marchev, A. S. The Untapped Potential of Hairy Root Cultures and Their Multiple Applications. Preprints2024, 2024101240. https://doi.org/10.20944/preprints202410.1240.v1
Mirmazloum, I.; Slavov, A. K.; Marchev, A. S. The Untapped Potential of Hairy Root Cultures and Their Multiple Applications. Preprints 2024, 2024101240. https://doi.org/10.20944/preprints202410.1240.v1
Mirmazloum, I.; Slavov, A. K.; Marchev, A. S. The Untapped Potential of Hairy Root Cultures and Their Multiple Applications. Preprints2024, 2024101240. https://doi.org/10.20944/preprints202410.1240.v1
APA Style
Mirmazloum, I., Slavov, A. K., & Marchev, A. S. (2024). The Untapped Potential of Hairy Root Cultures and Their Multiple Applications. Preprints. https://doi.org/10.20944/preprints202410.1240.v1
Chicago/Turabian Style
Mirmazloum, I., Aleksandar K. Slavov and Andrey S. Marchev. 2024 "The Untapped Potential of Hairy Root Cultures and Their Multiple Applications" Preprints. https://doi.org/10.20944/preprints202410.1240.v1
Abstract
Plants are rich source of specialized metabolites, such as alkaloids, terpenes, phenolic acids, flavonoids, coumarins, and volatile oils, which provide various health benefits including anticancer, anti-inflammatory, antiaging, skin-altering and anti-diabetic properties. However, challenges such as low and inconsistent yields, environment and geographic factors, and species-specific production of some specialized metabolites limit the supply of raw plant material for the food, cosmetic or pharmaceutical industries. Therefore, biotechnological approaches using plant in vitro systems offer an appealing alternative for the production of biologically active metabolites. Among these, hairy root cultures induced by the Rhizobium rhizogenes have firmed up their position as “green cell factories” due to their genotypic and biosynthetic stability. Hairy roots are valuable platforms for producing high-value phytomolecules at low cost, are amenable to pathway engineering and can be scaled up in bioreactors making them attractive for commercialization. This review explores the potential of hairy roots for specialized metabolites biosynthesis focusing on biotechnology tools to enhance their production. Aspects of morphological peculiarities of hairy roots, the diversity of bioreactors design, and process intensification technologies for maximizing biosynthetic capacity, as well as examples of patented plant-derived (green-labeled) products produced through hairy root cultivation at lab and industrial scales are addressed and discussed.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
