1. Introduction
The preservation of ecological balance and agricultural productivity is critically threatened by the spread of plant diseases and invasive species. These issues are not confined to specific geographic regions; they are global in scope and impact. According to the Food and Agriculture Organization (FAO), pests and diseases are responsible for up to 40% of global food crop losses annually, and invasive species can lead to losses in biodiversity that severely destabilize ecosystems [
1]. As ecosys-tems around the world become increasingly vulnerable due to climate change and human activity, early detection and intervention are necessary to mitigate these risks [
2]. However, traditional methods of identifying plant diseases and invasive species, which often rely on visual inspection by experts, are not scalable across large regions and fail to provide timely interventions [
3].
Recent advancements in machine learning and image processing techniques provide an oppor-tunity to address these challenges through automation. Image-based plant disease diagnosis has been the subject of extensive research in the field of precision agriculture, with convolutional neural networks (CNNs) showing great promise in accurately identifying diseased leaves [
16]. Moreover, morphological analysis has long been used to differentiate plant species and detect abnormalities at the microscopic level [
5]. However, the combination of both disease detection and invasive species identification in one unified computational model is relatively unexplored in the current literature. This paper seeks to address this gap by proposing a neural network-based model capable of de-tecting leaf diseases and identifying invasive species simultaneously, leveraging a suite of image processing techniques such as edge detection, color analysis, and feature extraction.
The approach taken in this research is guided by the hypothesis that integrating leaf color and shape information with neural network architectures can provide highly accurate detection models. By using machine learning algorithms, particularly neural networks, I can automate the process of leaf disease diagnosis and species identification, reducing the reliance on manual, labor-intensive inspection [
6]. Additionally, the use of edge detection techniques, such as Sobel and Canny filters, can significantly enhance the accuracy of shape detection, which is essential in identifying leaf margins and diseases [
20].
This paper aims to contribute to the growing body of research on ecological preservation by presenting a scalable, efficient, and reliable computational solution. The model I propose not only identifies diseases in plants but also assesses whether a leaf belongs to an invasive species, thus offering a dual-purpose tool for researchers and environmentalists. The outcomes of this study could significantly impact environmental conservation efforts by facilitating the early detection of invasive species, which is crucial for maintaining biodiversity [
17]. Furthermore, this research also aims to contribute to agricultural sustainability by enabling early intervention in plant disease outbreaks, potentially reducing crop losses and enhancing food security.
4. Methodology
The proposed methodology for plant disease detection and invasive species identification leverages a combination of image processing techniques and machine learning models, primarily Convolutional Neural Networks (CNNs). This section outlines the key stages of my approach, including dataset preparation, image pre-processing, feature extraction, model architecture, and evaluation metrics. Additionally, I describe the implementation details and provide relevant code snippets.
4.1. Dataset Preparation
My study utilized a diverse dataset consisting of both diseased and healthy plant leaves, as well as images of invasive and non-invasive plant species. The images were sourced from publicly available repositories, including the PlantVillage dataset and USDA’s plant disease and invasive species databases. The dataset was divided into training, validation, and test sets, with an 80-10-10 split. Each image was annotated with its respective class label, which was crucial for training supervised learning models.
To balance the dataset, I applied data augmentation techniques, including random rotations, flips, scaling, and translations, to increase the diversity of training samples. These transforma-tions were performed using the Python library imgaug. The following code snippet illustrates the augmentation process:
4.2. Image Pre-processing
Before feeding the images into the CNN model, I performed several pre-processing steps to enhance the feature extraction process. First, all images were resized to a fixed resolution of 224×224 pixels to ensure uniformity across the dataset. Then, pixel values were normalized to the range [0, 1] to accelerate model convergence during training. Additionally, color space conversion to HSV (Hue, Saturation, Value) was used for improved disease detection, particularly when symptoms manifest through color changes in leaves.
I also applied edge detection algorithms to emphasize leaf margins, which are essential for species identification. The following code demonstrates the use of the Sobel filter for edge detection:
4.3. Feature Extraction
Feature extraction was accomplished using CNNs, where each convolutional layer extracted spatial hierarchies of features such as edges, textures, and shapes. The initial layers of the CNN captured low-level features, while deeper layers abstracted higher-level patterns that were crucial for distin-guishing between healthy and diseased plants, as well as identifying species-specific morphological traits.
In addition to CNN-based feature extraction, I employed texture analysis techniques using Gray-Level Co-occurrence Matrix (GLCM) to capture texture-related features in leaf images. GLCM is widely used for analyzing variations in pixel intensities, making it particularly useful for disease symptom identification. The following code snippet illustrates the extraction of GLCM features:
4.4. Model Architecture
I adopted a deep CNN model based on the VGG16 architecture due to its proven effectiveness in image classification tasks. The model comprises multiple convolutional layers followed by ReLU (Rectified Linear Unit) activation functions and max-pooling layers. The output of the convolu-tional blocks is flattened and passed through fully connected layers, culminating in a softmax layer for multi-class classification.
The model was trained using the Adam optimizer with a learning rate of 0.001 and categorical cross-entropy as the loss function. Dropout layers with a 50% dropout rate were included to prevent overfitting. The following code demonstrates the CNN architecture using Keras:
4.5. Training and Evaluation
The model was trained for 50 epochs with a batch size of 32 on an NVIDIA Tesla V100 GPU. Early stopping and model checkpointing were employed to halt training once the validation loss stopped improving. The evaluation was conducted on the test set using accuracy, precision, recall, and F1-score as performance metrics. These metrics provided a comprehensive assessment of the model’s ability to distinguish between plant diseases and invasive species.
Additionally, I performed K-fold cross-validation (with K = 5) to ensure the model’s robustness and generalizability across different subsets of the data. The following code snippet demonstrates the implementation of K-fold cross-validation using scikit-learn:
4.6. Edge Detection for Leaf Margins
Edge detection, particularly using the Sobel and Canny filters, was crucial for identifying leaf margins and shapes, which are important features for species identification. The Sobel filter detects edges by calculating the gradient of image intensity at each pixel, while the Canny filter applies a multi-stage process to detect edges more robustly, especially in noisy images. The following code demonstrates the application of the Canny edge detection algorithm:
4.7. Integration of Invasive Species Detection
To incorporate invasive species detection into my leaf disease identification model, I extended the feature extraction process to account for the morphological characteristics of leaves commonly associated with invasive species. This included parameters like leaf shape, size, and margin char-acteristics, in addition to the disease detection features.
The invasive species detection was handled by expanding the classification task of my convolu-tional neural network (CNN). Initially designed to identify healthy or diseased leaves, the model was retrained to classify leaves into three categories: healthy, diseased, and invasive. The invasive species dataset was carefully curated with leaves known to exhibit invasive characteristics, allowing the model to learn both pixel-level distinctions and broader morphological traits.
Here is a snippet of the Python code that demonstrates how the CNN was adapted to handle invasive species classification:
In the above code, the CNN is trained to classify images of leaves as healthy, diseased, or invasive. The feature extraction process was designed to capture both low-level pixel details and high-level morphological features critical to identifying invasive species. By adding this functionality, the model can now detect whether a plant is an invasive species in addition to determining if it is diseased.
I evaluated the performance of invasive species detection using standard classification metrics such as accuracy, precision, recall, and F1-score. The results demonstrated a high level of accuracy in distinguishing between native and invasive species, which is crucial for managing ecological impacts.
5. Analysis
The evaluation of the leaf disease and invasive species detection algorithm focused on key perfor-mance metrics, particularly highlighting areas of success and potential improvement in identifying invasive species. The analysis draws attention to the model’s strengths, the challenges posed by data imbalance, and opportunities for enhancement.
5.1. Performance Evaluation Metrics
The classification performance for each category (healthy, diseased, and invasive) was assessed using the following metrics:
Accuracy: Measures overall correctness across all categories.
Precision: Indicates the proportion of true positive predictions relative to all positive pre-dictions.
Recall (Sensitivity): Reflects the model’s ability to capture true positives among all actual positive cases.
F1-Score: Combines precision and recall, providing a balanced measure of the model’s ef-fectiveness, especially when dealing with imbalanced datasets.
Table 1.
Performance Evaluation Metrics for Leaf Detection and Classification.
Table 1.
Performance Evaluation Metrics for Leaf Detection and Classification.
| Category |
Accuracy (%) |
Precision (%) |
Recall (%) |
F1-Score (%) |
| |
|
|
|
|
| Healthy |
98.2 |
97.8 |
98.4 |
98.1 |
| |
|
|
|
|
| Diseased |
95.7 |
95.3 |
95.9 |
95.6 |
| |
|
|
|
|
| Invasive |
92.1 |
91.7 |
92.4 |
92.0 |
| |
|
|
|
|
Results indicated strong performance in detecting healthy and diseased leaves, while the invasive species detection exhibited lower precision and recall. This discrepancy highlights the inherent challenge of distinguishing invasive species based on their subtle morphological differences from native or diseased species.
5.2. Invasive Species Detection Performance
Invasive species detection proved to be the most challenging task for the model. With an accuracy of 92.1%, precision and recall metrics slightly lagged behind those of the healthy and diseased categories, primarily due to class imbalance and morphological similarities between invasive species and native plants.
Several invasive species were particularly difficult to identify, such as Phragmites australis and Ailanthus altissima, which closely resemble some native species or those suffering from certain diseases. Despite these challenges, data augmentation strategies, including rotation and scaling, helped mitigate the issue to some extent by enriching the training data for invasive species.
5.3. Addressing Class Imbalance in Invasive Species Detection
One of the primary factors contributing to the reduced accuracy in detecting invasive species was the imbalance in the dataset, where invasive species were underrepresented. To address this issue, I employed several strategies:
Data augmentation: I applied transformations such as flipping, rotation, and scaling to artificially inflate the dataset of invasive species, allowing the model to learn from more diverse examples.
Transfer learning: Fine-tuning a pre-trained model helped improve feature extraction, as the base model had already been trained on a large, diverse image set, which gave it an advantage in detecting the finer details that distinguish invasive species.
The result was an increase in detection accuracy, though further improvement could still be made by collecting more invasive species data and refining the feature extraction process to focus on distinct morphological traits.
5.4. Cross-Validation and Generalization Capability
To ensure the model’s robustness, I used k-fold cross-validation (with k = 5) to assess its gen-eralization ability. The model consistently achieved strong performance across the folds, with an average accuracy of 93.5%, which validates its ability to handle unseen data effectively.
Moreover, when tested on an external dataset, the model demonstrated similar levels of accuracy, particularly in detecting healthy and diseased leaves. However, invasive species detection continued to lag, indicating a need for further refinement in detecting these subtle morphological cues in real-world applications.
5.5. Hyperparameter Tuning and Model Optimization
Hyperparameter tuning played a critical role in improving the model’s performance. Grid search optimization helped identify the best combination of learning rate, dropout rate, and batch size, which significantly impacted the model’s ability to generalize without overfitting. The optimal combination (learning rate: 0.0001, dropout rate: 0.5) provided a balance between training stability and performance on the test set.
5.6. Error Analysis and Future Directions
While the model excelled at detecting diseases and healthy leaves, further improvements are needed for invasive species detection. Future research will focus on expanding the dataset, particularly for underrepresented invasive species, and refining the feature extraction pipeline to emphasize traits specific to invasive species.
Integrating additional biological information, such as environmental factors or leaf venation patterns, may improve the model’s capacity to accurately differentiate between invasive species and diseases. Moreover, incorporating higher-resolution images or multispectral data could provide new avenues for detecting subtle differences in leaf morphology.