Li, H.; Liu, L.; Kong, X.; Wang, X.; Si, A.; Zhao, F.; Huang, Q.; Yu, Y.; Chen, Z. Time-Course Transcriptomics Analysis Reveals Molecular Mechanisms of Salt-Tolerant and Salt-Sensitive Cotton Cultivars to Salt Stress. Preprints2024, 2024101350. https://doi.org/10.20944/preprints202410.1350.v1
APA Style
Li, H., Liu, L., Kong, X., Wang, X., Si, A., Zhao, F., Huang, Q., Yu, Y., & Chen, Z. (2024). Time-Course Transcriptomics Analysis Reveals Molecular Mechanisms of Salt-Tolerant and Salt-Sensitive Cotton Cultivars to Salt Stress. Preprints. https://doi.org/10.20944/preprints202410.1350.v1
Chicago/Turabian Style
Li, H., Yu Yu and Zhiwen Chen. 2024 "Time-Course Transcriptomics Analysis Reveals Molecular Mechanisms of Salt-Tolerant and Salt-Sensitive Cotton Cultivars to Salt Stress" Preprints. https://doi.org/10.20944/preprints202410.1350.v1
Abstract
Salt stress is an environmental factor that limits plant seed germination, growth, and survival. We performed a comparative RNA sequencing transcriptome analysis of the seeds during germination from two cultivars with contrasting salt tolerance responses. A transcriptomic comparison between salt-tolerant cotton cv Jin-mian 25 and salt-sensitive cotton cv Su-mian 3 revealed both similar and differential expression patterns between the two genotypes during salt stress. The expression of genes related to aquaporins, kinases, reactive oxygen species (ROS) scavenging, trehalose biosynthesis, and phytohormone biosynthesis and signaling that include ethylene (ET), gibberellin (GA), abscisic acid (ABA), jasmonic acid (JA), and brassinosteroid (BR) were systematically investigated between the cultivars. Despite the involvement of these genes in cotton's response to salt stress in positive or negative ways, their expression levels were mostly similar in both genotypes. Interestingly, a PXC2 gene (Ghir_D08G025150) was identified, which encodes a leucine-rich repeat receptor-like protein kinase (LRR-RLK). This gene showed an induced expression pattern after salt stress treatment only in salt-tolerant cv Jin-mian 25, but not salt-sensitive cv Su-mian 3. Our multifaceted transcriptome approach illustrated a differential response to salt stress between salt-tolerant and salt-sensitive cotton.
Keywords
Salt Stress
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
