Preprint Article Version 1 This version is not peer-reviewed

Phytochemical Characterization, Bioactive Properties, and Acute Toxicity of Wild Vitex agnus-castus L. methanolic extracts

Radhia Mejri
,
Reyadh R Al Rashidi
,
Yosra Hasni
,
Aicha Laouani
,
Syrine Chelly
,
Hamza ELFEKIH
,
Oumayma Ben Rejeb
,
Mouna Kouira
,
Ghada SAAD
,
Amira Zaïri
*
Version 1 : Received: 16 October 2024 / Approved: 16 October 2024 / Online: 17 October 2024 (17:02:18 CEST)

How to cite: Mejri, R.; Al Rashidi, R. R.; Hasni, Y.; Laouani, A.; Chelly, S.; ELFEKIH, H.; Ben Rejeb, O.; Kouira, M.; SAAD, G.; Zaïri, A. Phytochemical Characterization, Bioactive Properties, and Acute Toxicity of Wild Vitex agnus-castus L. methanolic extracts. Preprints 2024, 2024101414. https://doi.org/10.20944/preprints202410.1414.v1 Mejri, R.; Al Rashidi, R. R.; Hasni, Y.; Laouani, A.; Chelly, S.; ELFEKIH, H.; Ben Rejeb, O.; Kouira, M.; SAAD, G.; Zaïri, A. Phytochemical Characterization, Bioactive Properties, and Acute Toxicity of Wild Vitex agnus-castus L. methanolic extracts. Preprints 2024, 2024101414. https://doi.org/10.20944/preprints202410.1414.v1

Abstract

Vitex agnus-castus L.(VAC) belonging to the Verbenaceae family is a Mediterranean plant which is widely used in traditional Tunisian medicine. The aim of this study was to investigate the phytochemical composition, antioxidant, antiviral and cytotoxic potential of methanolic VAC fruits extracts. Quantitative analyses of the different constituents of extracts were evaluated by high-performance liquid chromatography with photodiode-array detection (HPLC-DAD). Spectrophotometric methods and chemical tests were used for phytochemical characterization and antioxidant activity. The cytotoxic study was evaluated using epidermoid carcinoma Hep-2 cells, while the antiviral assessment against clinical isolates of coxsackievirus B3 (CBV-3) was determined by using a HEp cell survival assay. VAC extracts presented several components and minerals The results of HPLC-DAD indicated the presence 7 of phenolic compounds with a remarkable abondance of chlorogenic and coumaric acids as polyphenols compounds. The data also showed that VAC extracts had an important antioxidant effect (IC50=0.061 mg/mL) with a reduced cytotoxicity (CC50= 213 µg/ml), and these effects are displayed in a dose-dependent manner. However, all the tested extracts are inactive against CBV-3. The results achieved confirm the important role of this plant as a source of therapeutic activities.

Keywords

Vitex agnus-castus L.; HPLC-DAD; antioxidant activity; antiviral activity; cytotoxic activity; HEp-2 cells

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.