Version 1
: Received: 21 October 2024 / Approved: 22 October 2024 / Online: 24 October 2024 (10:02:13 CEST)
How to cite:
He, J.; Zhou, H.; Liang, J.; Tuerxun, K.; Ding, Z.; Zhou, S. HOM2 Deletion by CRISPR-Cas9 in Saccharomyces cerevisiae for decreasing Higher Alcohols in Whiskey. Preprints2024, 2024101702. https://doi.org/10.20944/preprints202410.1702.v1
He, J.; Zhou, H.; Liang, J.; Tuerxun, K.; Ding, Z.; Zhou, S. HOM2 Deletion by CRISPR-Cas9 in Saccharomyces cerevisiae for decreasing Higher Alcohols in Whiskey. Preprints 2024, 2024101702. https://doi.org/10.20944/preprints202410.1702.v1
He, J.; Zhou, H.; Liang, J.; Tuerxun, K.; Ding, Z.; Zhou, S. HOM2 Deletion by CRISPR-Cas9 in Saccharomyces cerevisiae for decreasing Higher Alcohols in Whiskey. Preprints2024, 2024101702. https://doi.org/10.20944/preprints202410.1702.v1
APA Style
He, J., Zhou, H., Liang, J., Tuerxun, K., Ding, Z., & Zhou, S. (2024). HOM2 Deletion by CRISPR-Cas9 in Saccharomyces cerevisiae for decreasing Higher Alcohols in Whiskey. Preprints. https://doi.org/10.20944/preprints202410.1702.v1
Chicago/Turabian Style
He, J., Zhuoling Ding and Shishui Zhou. 2024 "HOM2 Deletion by CRISPR-Cas9 in Saccharomyces cerevisiae for decreasing Higher Alcohols in Whiskey" Preprints. https://doi.org/10.20944/preprints202410.1702.v1
Abstract
In typical whiskey, the content of higher alcohols is about 1500-2000 mg/L, lead to high intoxicating degree (ID). To produce the low-ID whiskey, the Saccharomyces cerevisiae XF0-h, XF0-H and XF0-LH were successfully constructed by CRISPR-Cas9 gene editing technology to knock out HOM2 (encoding aspartate β-semialdehyde dehydrogenase) in the original strain XF0 and the LEU1 knockout strain XF0-L. The content of higher alcohols in whiskey fermented by the XF0-h, XF0-H, and XF0-LH were 704±8 mg/L, 685±6 mg/L, and 685±19 mg/L, respectively, showing reductions of 23.93%, 25.98%, and 15.81% compared to the XF0, XF0, and XF0-L. The fermentation conditions of the XF0-LH were optimized through single-factor experiments and Box-Behnken design. The optimal conditions were the wort concentration of 9.8 °P, the hydrolyzed broken rice syrup addition of 78 g/L, and the inoculum size of 2.7 × 106 cells/mL. The low-ID whiskey was brewed with higher alcohols content at 556 mg/L by 50L fermenter at the optimal conditions.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
