Version 1
: Received: 22 October 2024 / Approved: 22 October 2024 / Online: 22 October 2024 (17:00:30 CEST)
How to cite:
Lee, E.-H.; Cho, J.; Kang, I. Photoaging Protective Effects of Green Ball Apple Peel Isolated Quercitrin. Preprints2024, 2024101750. https://doi.org/10.20944/preprints202410.1750.v1
Lee, E.-H.; Cho, J.; Kang, I. Photoaging Protective Effects of Green Ball Apple Peel Isolated Quercitrin. Preprints 2024, 2024101750. https://doi.org/10.20944/preprints202410.1750.v1
Lee, E.-H.; Cho, J.; Kang, I. Photoaging Protective Effects of Green Ball Apple Peel Isolated Quercitrin. Preprints2024, 2024101750. https://doi.org/10.20944/preprints202410.1750.v1
APA Style
Lee, E. H., Cho, J., & Kang, I. (2024). Photoaging Protective Effects of Green Ball Apple Peel Isolated Quercitrin. Preprints. https://doi.org/10.20944/preprints202410.1750.v1
Chicago/Turabian Style
Lee, E., Junhyo Cho and Inkyu Kang. 2024 "Photoaging Protective Effects of Green Ball Apple Peel Isolated Quercitrin" Preprints. https://doi.org/10.20944/preprints202410.1750.v1
Abstract
Premature skin aging, also known as photoaging, refers to the changes of the structure and function of the skin caused by chronic sun exposure. The ultraviolet radiation in the sunlight is one of the key factors that causes photoaging. Thus, MMPs, TGFB1, and NF-κB signaling can be an effective therapeutic strategy for UVB exposure. In this study, we used human dermal fibroblast and mouse macrophage cells to identify the mediators of skin photoaging. Quercitrin isolated from ‘Green Ball’ apple peel was treated to UVB-irradiated fibroblast cell and lipopolysaccharide (LPS)-induced macrophages to identify photoaging prevention effect of quercitrin. Genes that are associated with photoaging was determined by using ELISA, western blot, and qPCR. Quercitrin increased collagen biosynthesis in UVB-irradiated fibroblast cell via regulating MMPs, TIMP-1, TGFB1, HAS2, and COL1A2. In addition, quercitrin regulated p-65, iNOS, COX-2, and mediators (PGE2 and NO), in the NF-κB signaling process, and inhibited the production of cytokines in LPS-induced macrophages. These results indicates that quercitrin can improve photoaging damaged skin by regulating MMPs, TGFβ, and NF-κB signaling pathway modulators.
Keywords
collagen; cytokine; matrix metalloproteinase; protein expression; real-time PCR
Subject
Biology and Life Sciences, Aging
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
