Version 1
: Received: 22 October 2024 / Approved: 22 October 2024 / Online: 23 October 2024 (11:39:07 CEST)
How to cite:
Raza, S.; Uçan, U.; Aksoy, M.; Erdoğan, G.; Naseer, Z.; Khan, K. Sericin-Enriched Rabbit Semen Preservation: Implications for Short-Term Storage Quality and Fertility at 4 or 15°C. Preprints2024, 2024101778. https://doi.org/10.20944/preprints202410.1778.v1
Raza, S.; Uçan, U.; Aksoy, M.; Erdoğan, G.; Naseer, Z.; Khan, K. Sericin-Enriched Rabbit Semen Preservation: Implications for Short-Term Storage Quality and Fertility at 4 or 15°C. Preprints 2024, 2024101778. https://doi.org/10.20944/preprints202410.1778.v1
Raza, S.; Uçan, U.; Aksoy, M.; Erdoğan, G.; Naseer, Z.; Khan, K. Sericin-Enriched Rabbit Semen Preservation: Implications for Short-Term Storage Quality and Fertility at 4 or 15°C. Preprints2024, 2024101778. https://doi.org/10.20944/preprints202410.1778.v1
APA Style
Raza, S., Uçan, U., Aksoy, M., Erdoğan, G., Naseer, Z., & Khan, K. (2024). Sericin-Enriched Rabbit Semen Preservation: Implications for Short-Term Storage Quality and Fertility at 4 or 15°C. Preprints. https://doi.org/10.20944/preprints202410.1778.v1
Chicago/Turabian Style
Raza, S., Zahid Naseer and Komal Khan. 2024 "Sericin-Enriched Rabbit Semen Preservation: Implications for Short-Term Storage Quality and Fertility at 4 or 15°C" Preprints. https://doi.org/10.20944/preprints202410.1778.v1
Abstract
The influence of sericin supplementation and chilling temperatures (4°C and 15°C) on the short-term storage quality and fertility of rabbit semen was assessed over 72 hours of storage. In experiment 1, pooled semen (from five ejaculates) was diluted to a concentration of 50M ml−1, and assigned to control, 0.1% and 0.5% sericin groups, stored at 4 or 15°C. Sperm motility, sperm kinematics, viability, membrane and acrosome integrity were assessed at 0, 24, 48, and 72 hours of storage. The experiment 2 and 3 were conducted to observe effect of sericin on bacterial growth and in vivo fertility. The results of experiment 1 showed that sericin treatment, storage temperature, and storage duration influenced progressive and total motility, as well as sperm kinematics. Similar effects were observed for acrosome integrity, membrane integrity, and sperm viability. The results of experiment 2 indicated the antimicrobial effects of sericin when sperm were stored at 15C for 72 hours. While promising pregnancy outcomes were observed with sericin treated sperm for 72 hours, these results not significantly different. The study conclusively demonstrates that sericin treatment enhances the quality of rabbit semen when stored at lower temperatures for longer durations. Antimicrobial effects of sericin could be a contributing factor to the improved in-vitro and in-vivo fertility of rabbit sperm.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
