Version 1
: Received: 24 October 2024 / Approved: 24 October 2024 / Online: 25 October 2024 (09:34:57 CEST)
How to cite:
Hernández-Moreno, A.; Nava-Tapia, D. A.; Zuñiga-Eulogio, M. D.; Bello-Martínez, J.; Olea-Flores, M.; Hernández-Moreno, T.; Ordoñez, M.; Zacapala-Gómez, A. E.; Mendoza-Catalán, M. A.; Navarro-Tito, N. Anti-Migratory Activity of Brazilin Chemo-Diversification Products on Breast Cancer Cells. Preprints2024, 2024101929. https://doi.org/10.20944/preprints202410.1929.v1
Hernández-Moreno, A.; Nava-Tapia, D. A.; Zuñiga-Eulogio, M. D.; Bello-Martínez, J.; Olea-Flores, M.; Hernández-Moreno, T.; Ordoñez, M.; Zacapala-Gómez, A. E.; Mendoza-Catalán, M. A.; Navarro-Tito, N. Anti-Migratory Activity of Brazilin Chemo-Diversification Products on Breast Cancer Cells. Preprints 2024, 2024101929. https://doi.org/10.20944/preprints202410.1929.v1
Hernández-Moreno, A.; Nava-Tapia, D. A.; Zuñiga-Eulogio, M. D.; Bello-Martínez, J.; Olea-Flores, M.; Hernández-Moreno, T.; Ordoñez, M.; Zacapala-Gómez, A. E.; Mendoza-Catalán, M. A.; Navarro-Tito, N. Anti-Migratory Activity of Brazilin Chemo-Diversification Products on Breast Cancer Cells. Preprints2024, 2024101929. https://doi.org/10.20944/preprints202410.1929.v1
APA Style
Hernández-Moreno, A., Nava-Tapia, D. A., Zuñiga-Eulogio, M. D., Bello-Martínez, J., Olea-Flores, M., Hernández-Moreno, T., Ordoñez, M., Zacapala-Gómez, A. E., Mendoza-Catalán, M. A., & Navarro-Tito, N. (2024). Anti-Migratory Activity of Brazilin Chemo-Diversification Products on Breast Cancer Cells. Preprints. https://doi.org/10.20944/preprints202410.1929.v1
Chicago/Turabian Style
Hernández-Moreno, A., Miguel A. Mendoza-Catalán and Napoleón Navarro-Tito. 2024 "Anti-Migratory Activity of Brazilin Chemo-Diversification Products on Breast Cancer Cells" Preprints. https://doi.org/10.20944/preprints202410.1929.v1
Abstract
Breast cancer is the most common and the leading cause of cancer death in women worldwide; treating invasive breast carcinomas is challenging due to the side effects of chemotherapeutics. Compounds isolated from natural sources have been proposed as potential molecules for cancer therapy; for instance, the homoisoflavonoid brazilin has shown pharmacological properties, including anti-tumoral and anti-inflammatory activities. In this study, we isolated brazilin from the heartwood of Haematoxylum brasiletto (Pacific coast of Mexico and Central America); then, we performed a semi-synthesis by adding three methyl or acetyl groups to the core structure of brazilin. We confirmed brazilin and its derivatives identity by spectroscopic data (1H NMR and 13C NMR) and measured their purity by optical rotation. Then, we analyzed the effects of brazilin and derivatives in three mammary gland-derived cell lines: the TNBC MDA-MB-231, the ER(+) MCF7, and the non-tumorigenic MCF10A. We evaluated the cell viability by MTT assays, cell migration by wound-healing assays and focal adhesion kinase (FAK) activation by Western blot. Regarding biological assays, the MTT assay showed that brazilin and its derivatives showed cytotoxic effects on MCF7 and MDA-MB-231 breast cancer cells at 20 µM but was not toxic in non-tumorigenic MCF10A mammary epithelial cells. These effects were dose and time-dependent, as well as a decrease in the levels of cell migration and FAK activation.
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
