Preprint Article Version 1 This version is not peer-reviewed

Bone Marrow Stem Cell Population in Single and Multiple Level Aspiration

Xiangguo Che
ORCID logo ,
Hee-June Kim
,
Xian Jin PhD
ORCID logo ,
Joon-Woo Kim
ORCID logo ,
Kyeong-Hyeon Park
ORCID logo ,
Jeong-Ok Lim
,
Hee-Soo Kyung
,
Chang-Wug Oh
* ORCID logo ,
Je-Yong Choi
* ORCID logo
Version 1 : Received: 23 October 2024 / Approved: 24 October 2024 / Online: 25 October 2024 (10:23:05 CEST)

How to cite: Che, X.; Kim, H.-J.; PhD, X. J.; Kim, J.-W.; Park, K.-H.; Lim, J.-O.; Kyung, H.-S.; Oh, C.-W.; Choi, J.-Y. Bone Marrow Stem Cell Population in Single and Multiple Level Aspiration. Preprints 2024, 2024101945. https://doi.org/10.20944/preprints202410.1945.v1 Che, X.; Kim, H.-J.; PhD, X. J.; Kim, J.-W.; Park, K.-H.; Lim, J.-O.; Kyung, H.-S.; Oh, C.-W.; Choi, J.-Y. Bone Marrow Stem Cell Population in Single and Multiple Level Aspiration. Preprints 2024, 2024101945. https://doi.org/10.20944/preprints202410.1945.v1

Abstract

Background: Bone marrow aspiration concentrate (BMAC) has garnered increasing interest due to its potential for healing musculoskeletal injuries. While the iliac crest remains a common harvest site, the aspiration technique’s efficacy in offering the highest yield and prevalence of mesen-chymal stem cells (MSCs) is controversial. This study aimed to compare two different techniques of bone marrow aspiration over the anterior iliac crest from a single level (SL) versus multiple level (ML). Methods: Anterior iliac crests were selected in 9 adult patients (aged between 31 and 59 years old). Aspiration was achieved using an 11-gauge needle (length: 100 mm; diameter: 2.3 mm) specifically manufactured for bone marrow collection (BD, Becton, US) connected to a 10-mL syringe. On one side, 4cc of bone marrow was aspirated at an SL to a depth of 7cm without changing the needle direction. On the other side, over the same portion of the iliac crest, 1 cc of bone marrow was obtained from ML of different depths during needle retrieval, maintaining a distance of 1cm and changing the tip direction. The samples were blindly sent to the laboratory without indicating whether they came from an SL or ML. Fluorescence-activated cell sorting (FACS) and osteoblast differentiation were analyzed and compared. Results: In the FACS analysis, the SL resulted in a higher population of MSCs that were positive for CD105, CD73, and CD90 and negative for CD34, compared to the ML method. In the process of osteoblast differentiation, it was observed that MSCs exhibited more advanced features of en-hanced osteoblastic abilities in the SL method rather than the ML method. Conclusion: An SL aspiration technique at the anterior iliac crest may produce a high-quality bone marrow aspirate. This technique may help obtain specific populations of MSCs with the desired characteristics for use in regenerative therapies for musculoskeletal injuries.

Keywords

Bone marrow aspiration concentrate; mesenchymal stem cells; single level; multiple level; musculoskeletal injuries

Subject

Medicine and Pharmacology, Other

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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