PreprintArticleVersion 1This version is not peer-reviewed
Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (Hermetia illucens) Larvae; Comparison to Mealworm (Tenebrio molitor) Larvae
Version 1
: Received: 31 October 2024 / Approved: 31 October 2024 / Online: 1 November 2024 (05:16:00 CET)
How to cite:
Lecocq, A.; Alencar, A. L. F.; Lazov, C. M.; Rajiuddin, S. M.; Bøtner, A.; Belsham, G. J. Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (Hermetia illucens) Larvae; Comparison to Mealworm (Tenebrio molitor) Larvae. Preprints2024, 2024102574. https://doi.org/10.20944/preprints202410.2574.v1
Lecocq, A.; Alencar, A. L. F.; Lazov, C. M.; Rajiuddin, S. M.; Bøtner, A.; Belsham, G. J. Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (Hermetia illucens) Larvae; Comparison to Mealworm (Tenebrio molitor) Larvae. Preprints 2024, 2024102574. https://doi.org/10.20944/preprints202410.2574.v1
Lecocq, A.; Alencar, A. L. F.; Lazov, C. M.; Rajiuddin, S. M.; Bøtner, A.; Belsham, G. J. Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (Hermetia illucens) Larvae; Comparison to Mealworm (Tenebrio molitor) Larvae. Preprints2024, 2024102574. https://doi.org/10.20944/preprints202410.2574.v1
APA Style
Lecocq, A., Alencar, A. L. F., Lazov, C. M., Rajiuddin, S. M., Bøtner, A., & Belsham, G. J. (2024). Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<em>Hermetia illucens</em>) Larvae; Comparison to Mealworm (<em>Tenebrio molitor</em>) Larvae. Preprints. https://doi.org/10.20944/preprints202410.2574.v1
Chicago/Turabian Style
Lecocq, A., Anette Bøtner and Graham J. Belsham. 2024 "Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<em>Hermetia illucens</em>) Larvae; Comparison to Mealworm (<em>Tenebrio molitor</em>) Larvae" Preprints. https://doi.org/10.20944/preprints202410.2574.v1
Abstract
Insect larvae production offers the potential for large-scale synthesis of high quality protein that can be used as feed or food. However, currently, there are limitations on the source of substrates for the insect larvae to use. One concern is the potential survival of animal pathogens within insect larvae if their feed is contaminated. In this study, the survival of a very stable virus, porcine parvovirus (PPV), within mealworm (Tenebrio molitor) and black soldier fly (BSF) (Hermetia illucens) larvae has been analyzed after oral ingestion of the virus. The PPV DNA genome could be readily detected by PCR in both types of larvae up until 9 days post ingestion (DPI), the end of the study period. Furthermore, infection of susceptible PK15 cells by PPV from homogenized mealworm larvae could be detected until at least 3 dpi, using an immunoperoxidase staining method, and up until 9 DPI using a more sensitive real time PCR assay. Thus, PPV can remain infectious within mealworm larvae during their main growth phase through to their harvesting. However, it may be considered that PPV is exceptional in this respect since it displays unusual stability, e.g. to heat.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
