Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Improving Interpretation Consistency of Serum Capillary Electrophoresis by Development of Quantitative Graphic Indexes

Jia-Ruei Yu
ORCID logo ,
Yu-Tan Wu
,
Yi-Juan Sung
,
Tzong-Shi Chiueh
,
Wei-Hsuan Yu
,
Feng-Nan Hwang
ORCID logo ,
Zong-Qi Wu
,
Zayd Anwar
,
Wan-Ying Lin
,
Hsin-Yao Wang
* ORCID logo
Version 1 : Received: 31 October 2024 / Approved: 1 November 2024 / Online: 1 November 2024 (09:54:02 CET)

How to cite: Yu, J.-R.; Wu, Y.-T.; Sung, Y.-J.; Chiueh, T.-S.; Yu, W.-H.; Hwang, F.-N.; Wu, Z.-Q.; Anwar, Z.; Lin, W.-Y.; Wang, H.-Y. Improving Interpretation Consistency of Serum Capillary Electrophoresis by Development of Quantitative Graphic Indexes. Preprints 2024, 2024110033. https://doi.org/10.20944/preprints202411.0033.v1 Yu, J.-R.; Wu, Y.-T.; Sung, Y.-J.; Chiueh, T.-S.; Yu, W.-H.; Hwang, F.-N.; Wu, Z.-Q.; Anwar, Z.; Lin, W.-Y.; Wang, H.-Y. Improving Interpretation Consistency of Serum Capillary Electrophoresis by Development of Quantitative Graphic Indexes. Preprints 2024, 2024110033. https://doi.org/10.20944/preprints202411.0033.v1

Abstract

Capillary zone electrophoresis-immunosubtraction (CZE-IS) is an essential laboratory test in diagnosing plasma cell neoplasms. However, current interpretation of the test results is subjective. To evaluate CZE-IS in a more precise manner, this study proposed five key indexes, namely sharpness index, light chain index, immunoglobulin G index, immunoglobulin A index, and immunoglobulin M index. The reference intervals of these indexes were established using CZE-IS curve data from a clinical laboratory of a referral medical center. A total of 1000 cases with normal electrophoretic patterns were sampled for reference interval establishment, and additional 20 cases were included for validation. The reference intervals in the γ zone were established: 1–6 (sharpness index), 1.06–2.71 (light chain index), 37–454 (immunoglobulin G index), (-9)–41 (immunoglobulin A index), and (-16)–46 (immunoglobulin M index). For the β2 zone, the reference intervals were 3–17 (sharpness index), 0.44–1.90 (light chain index), (-7)–61 (immunoglobulin G index), 2–117 (immunoglobulin A index), and (-12)–35 (immunoglobulin M index). The diagnostic performance of reference intervals of the proposed indexes in validation ranged from 95% to 100%. CZE-IS indexes provide the objective quantification of key characteristics in CZE-IS curves, and improve the precision of CZE-IS interpretation.

Keywords

Capillary Zone Electrophoresis; Monoclonal Gammopathy; Reference Interval

Subject

Medicine and Pharmacology, Hematology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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