Preprint Article Version 1 This version is not peer-reviewed

Isolation, Expansion and Characterization of Rat Hair Follicle Stem Cells

Patrícia Sousa
,
Bruna Lopes
,
Ana Catarina Sousa
,
André Coelho
,
Alícia de Sousa Moreira
,
Alexandra Rêma
,
Maria Gonçalves-Maia
,
Irina Amorim
,
Rui Alvites
,
Nuno Alves
,
Stefano Geuna
,
Ana Colette Maurício
*
Version 1 : Received: 31 October 2024 / Approved: 1 November 2024 / Online: 1 November 2024 (09:44:41 CET)

How to cite: Sousa, P.; Lopes, B.; Sousa, A. C.; Coelho, A.; Moreira, A. D. S.; Rêma, A.; Gonçalves-Maia, M.; Amorim, I.; Alvites, R.; Alves, N.; Geuna, S.; Maurício, A. C. Isolation, Expansion and Characterization of Rat Hair Follicle Stem Cells. Preprints 2024, 2024110035. https://doi.org/10.20944/preprints202411.0035.v1 Sousa, P.; Lopes, B.; Sousa, A. C.; Coelho, A.; Moreira, A. D. S.; Rêma, A.; Gonçalves-Maia, M.; Amorim, I.; Alvites, R.; Alves, N.; Geuna, S.; Maurício, A. C. Isolation, Expansion and Characterization of Rat Hair Follicle Stem Cells. Preprints 2024, 2024110035. https://doi.org/10.20944/preprints202411.0035.v1

Abstract

Stem cells are capable of self-renewal and differentiation into various specialized cells, making them a potential therapeutic option in regenerative medicine. This study establishes a comprehensive methodology for isolating, culturing, and characterizing rat hair follicle stem cells. Hair follicles were harvested from Sprague–Dawley rats and subjected to two different isolation techniques. Immunohistochemical analysis and real-time PCR confirm the expression of specific surface markers and genes, validating the cells' identity. Growth kinetics, colony formation units (CFU) and tri-differentiation capacity were also assessed. Additionally, the cells secretome was furthermore analyzed regarding its content in biofactors with wound healing properties. These findings highlight the potential of these cells as a valuable cell source for skin regeneration applications. They contribute to advancing our understanding of stem cell applications in regenerative medicine and hold promise for therapeutic interventions in various clinical contexts, aligning with broader research on the diverse capabilities of hair follicle stem cells.

Keywords

hair follicle stem cells; regenerative medicine; stem cell characterization; stem cell isolation; wound healing

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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