Version 1
: Received: 1 November 2024 / Approved: 1 November 2024 / Online: 1 November 2024 (18:54:24 CET)
How to cite:
Pereira, D. R.; Pérez-Betancourt, Y.; Távora, B. C. L. F.; Magalhaes, G. S.; Carmona-Ribeiro, A. M.; Faquim-Mauro, E. L. The Role of Dendritic Cells in Adaptive Immune Response Induced by Ova/Pdda Nanoparticles. Preprints2024, 2024110114. https://doi.org/10.20944/preprints202411.0114.v1
Pereira, D. R.; Pérez-Betancourt, Y.; Távora, B. C. L. F.; Magalhaes, G. S.; Carmona-Ribeiro, A. M.; Faquim-Mauro, E. L. The Role of Dendritic Cells in Adaptive Immune Response Induced by Ova/Pdda Nanoparticles. Preprints 2024, 2024110114. https://doi.org/10.20944/preprints202411.0114.v1
Pereira, D. R.; Pérez-Betancourt, Y.; Távora, B. C. L. F.; Magalhaes, G. S.; Carmona-Ribeiro, A. M.; Faquim-Mauro, E. L. The Role of Dendritic Cells in Adaptive Immune Response Induced by Ova/Pdda Nanoparticles. Preprints2024, 2024110114. https://doi.org/10.20944/preprints202411.0114.v1
APA Style
Pereira, D. R., Pérez-Betancourt, Y., Távora, B. C. L. F., Magalhaes, G. S., Carmona-Ribeiro, A. M., & Faquim-Mauro, E. L. (2024). The Role of Dendritic Cells in Adaptive Immune Response Induced by Ova/Pdda Nanoparticles. Preprints. https://doi.org/10.20944/preprints202411.0114.v1
Chicago/Turabian Style
Pereira, D. R., Ana Maria Carmona-Ribeiro and Eliana L. Faquim-Mauro. 2024 "The Role of Dendritic Cells in Adaptive Immune Response Induced by Ova/Pdda Nanoparticles" Preprints. https://doi.org/10.20944/preprints202411.0114.v1
Abstract
Cationic polymers were previously shown to assemble with negatively charged proteins yielding nanoparticles (NPs). Poly-diallyl-dimethyl-ammonium chloride (PDDA) is a cationic polymer able to combine with ovalbumin (OVA) yielding a stable colloidal dispersion of OVA/PDDA NPs eliciting significant anti-OVA immune response. Dendritic Cells (DC), as sentinels of foreign antigens, exert a crucial role in the induction of antigen-specific T cell activation and consequent adaptive immune response. Objective: The present study aimed at evaluating the involvement of DCs in the adaptive immune response induced by OVA/PDDA. Methods/Results: Confirming the potent induction of adaptive immune response against OVA/PDDA, the data showed increased CD19+CD138+ plasma cells and CD19+CD38+CD27+ memory cells in spleens of mice immunized with OVA/PDDA-NPs 28 days before. OVA/PDDA-NPs also induced the migration and maturation of DCs to draining lymph nodes on days 3 and 4 of mice immunization. The in vitro results with bone-marrow differentiated DCs (iBM-DCs) showed an increase of the binding and uptake of OVA/PDDA NPs by these cells compared with soluble OVA. In addition, OVA/PDDA NPs were able to induce DC maturation and upregulation of costimulatory and MHC-II molecules, TNF- and IL-12 production. iBM-DCs incubated with OVA/PDDA NPs promoted high OVA-specific T cell proliferative response. Conclusion: Altogether, the data demonstrated the central role of DCs in the induction of antigen-specific immune response by OVA-PDDA-NPs, thus proving these NPs as potent adjuvants for subunit vaccine design.
Public Health and Healthcare, Public Health and Health Services
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
