Preprint Article Version 1 This version is not peer-reviewed

The Cellular and Transcriptomic Early Innate Immune Response to BCG Vaccination in Mice

Liya G. Kondratyeva
* ORCID logo ,
Olga A. Rakitina
ORCID logo ,
Victor V. Pleshkan
ORCID logo ,
Alexey I. Kuzmich
ORCID logo ,
Irina A. Linge
ORCID logo ,
Sofia A. Kondratieva
,
Eugene V. Snezhkov
,
Irina V. Alekseenko
,
Eugene D. Sverdlov
Version 1 : Received: 31 October 2024 / Approved: 2 November 2024 / Online: 4 November 2024 (04:50:45 CET)

How to cite: Kondratyeva, L. G.; Rakitina, O. A.; Pleshkan, V. V.; Kuzmich, A. I.; Linge, I. A.; Kondratieva, S. A.; Snezhkov, E. V.; Alekseenko, I. V.; Sverdlov, E. D. The Cellular and Transcriptomic Early Innate Immune Response to BCG Vaccination in Mice. Preprints 2024, 2024110136. https://doi.org/10.20944/preprints202411.0136.v1 Kondratyeva, L. G.; Rakitina, O. A.; Pleshkan, V. V.; Kuzmich, A. I.; Linge, I. A.; Kondratieva, S. A.; Snezhkov, E. V.; Alekseenko, I. V.; Sverdlov, E. D. The Cellular and Transcriptomic Early Innate Immune Response to BCG Vaccination in Mice. Preprints 2024, 2024110136. https://doi.org/10.20944/preprints202411.0136.v1

Abstract

It is established that BCG vaccination results in the development of both a specific immune response to mycobacterial infections and a nonspecific (heterologous) immune response, designated as trained immunity (TRIM), to other pathogens. We hypothesized that local BCG immunization may induce early immune response of bone marrow and spleen innate immunity cells. The early transcriptomic response of various populations of innate immune cells, including monocytes, neutrophils, and natural killer (NK) cells, to BCG vaccination was examined. To this end, C57Bl/6J mice were subcutaneously immunized with 10⁶ CFU of BCG. Three days following BCG administration, the three cell populations were collected from the control and BCG-vaccinated groups by FACS. All cell populations obtained were utilized for the preparation and sequencing of RNA-seq libraries. The analysis of FACS data revealed an increase in the proportion of splenic NK cells and monocytes 3 days post-vaccination. Transcriptomic analysis revealed deregulation of genes associated with regulation of immune response (according to Gene Ontology terms) in NK cells, monocytes, and unsorted bone marrow. Two NK cell-specific immune ligands (Tnfsf14 and S100a8) and two bone marrow-specific immune receptors (C5ar1 and Csf2rb) were identified among differentially expressed genes. No alterations were identified in neutrophils in either the percentage or at the transcriptomic level. Thus, in this study we demonstrated that BCG vaccination provides the early increase in the proportion of murine bone marrow and spleen immune cell populations, as well as the transcriptomic alterations in monocytes, NK cells, and non-sorted bone marrow. This early innate immune response may be beneficial for enhancing TRIM.

Keywords

trained immunity; BCG; innate immunity; vaccination; transcriptome; RNA-seq

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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