Version 1
: Received: 4 November 2024 / Approved: 6 November 2024 / Online: 7 November 2024 (02:05:17 CET)
How to cite:
Doan, T. N. A.; Lin, Y.-W. Electroacupuncture Mitigates TRPV1 Overexpression in the Central Nervous System Associated with Fibromyalgia in Mice. Preprints2024, 2024110417. https://doi.org/10.20944/preprints202411.0417.v1
Doan, T. N. A.; Lin, Y.-W. Electroacupuncture Mitigates TRPV1 Overexpression in the Central Nervous System Associated with Fibromyalgia in Mice. Preprints 2024, 2024110417. https://doi.org/10.20944/preprints202411.0417.v1
Doan, T. N. A.; Lin, Y.-W. Electroacupuncture Mitigates TRPV1 Overexpression in the Central Nervous System Associated with Fibromyalgia in Mice. Preprints2024, 2024110417. https://doi.org/10.20944/preprints202411.0417.v1
APA Style
Doan, T. N. A., & Lin, Y. W. (2024). Electroacupuncture Mitigates TRPV1 Overexpression in the Central Nervous System Associated with Fibromyalgia in Mice. Preprints. https://doi.org/10.20944/preprints202411.0417.v1
Chicago/Turabian Style
Doan, T. N. A. and Yi-Wen Lin. 2024 "Electroacupuncture Mitigates TRPV1 Overexpression in the Central Nervous System Associated with Fibromyalgia in Mice" Preprints. https://doi.org/10.20944/preprints202411.0417.v1
Abstract
Background: Fibromyalgia (FM) is characterized by chronic pain, significantly affecting the quality of life and functional capabilities of patients. In addition to pain, patients may experience insomnia, chronic fatigue, depression, anxiety, and headaches, further complicating their overall well-being. The Transient Receptor Potential Vanilloid 1 (TRPV1) receptor responds to various noxious stimuli and plays a key role in regulating pain sensitivity and inflammation. Thus, targeting TRPV1 may provide analgesic and anti-inflammatory benefits. This study investigates the efficacy of electroacupuncture (EA) in alleviating chronic pain in FM through TRPV1 and its downstream molecules in central nervous system (CNS). Methods: To model FM, we subjected mice to intermittent cold stress (ICS) for three days. The study comprised five rodent groups: Control, Intermitten cold stress induced FM (ICS), ICS + Electroacupuncture (EA), ICS + Sham Electroacupuncture (sham EA), and ICS + KO (TRPV1 knockout mice). Results: Our findings revealed that ICS induced allodynia and hyperalgesia in mice by day four, persisting until day 21. EA at 2 Hz and TRPV1 knockout significantly decreased both mechanical and thermal hypersensitivity (Withdrawal - Day 14: 2.43 ± 0.19 g; Day 21: 5.88 ± 0.47 g, n = 6, p < 0.05; Latency - Day 14: 2.77 ± 0.22 s; Day 21: 5.85 ± 0.41 s, n = 6, p < 0.05). In contrast, sham EA did not produce significant effects. Additionally, TRPV1 and several pain-related proteins were significantly elevated in the thalamus, somatosensory cortex (SSC), medial prefrontal cortex (mPFC), hippocampus, hypothalamus, cerebellum regions V (CB V), VI (CB VI) and VII (CB VII) after ICS model. Both EA at ST36 acupoint and TRPV1 knockout mice diminished overexpression of pain-related proteins, with the sham EA group showing no significant changes compared to the ICS group. Conclusion: Chronic widespread pain was reduced by EA and TRPV1 knockout, with the effects of EA on the TRPV1 pain pathway clearly evident in CNS after 21 days.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
