Version 1
: Received: 6 November 2024 / Approved: 6 November 2024 / Online: 8 November 2024 (04:00:33 CET)
How to cite:
Heatlie, J. K.; Lazniewska, J.; Moore, C. R.; Johnson, I. R.; Nturubika, B. D.; Williams, R.; Ward, M. P.; O'Leary, J. J.; Butler, L. M.; Brooks, D. A. Prostate Cancer Intercellular Communication. Preprints2024, 2024110469. https://doi.org/10.20944/preprints202411.0469.v1
Heatlie, J. K.; Lazniewska, J.; Moore, C. R.; Johnson, I. R.; Nturubika, B. D.; Williams, R.; Ward, M. P.; O'Leary, J. J.; Butler, L. M.; Brooks, D. A. Prostate Cancer Intercellular Communication. Preprints 2024, 2024110469. https://doi.org/10.20944/preprints202411.0469.v1
Heatlie, J. K.; Lazniewska, J.; Moore, C. R.; Johnson, I. R.; Nturubika, B. D.; Williams, R.; Ward, M. P.; O'Leary, J. J.; Butler, L. M.; Brooks, D. A. Prostate Cancer Intercellular Communication. Preprints2024, 2024110469. https://doi.org/10.20944/preprints202411.0469.v1
APA Style
Heatlie, J. K., Lazniewska, J., Moore, C. R., Johnson, I. R., Nturubika, B. D., Williams, R., Ward, M. P., O'Leary, J. J., Butler, L. M., & Brooks, D. A. (2024). Prostate Cancer Intercellular Communication. Preprints. https://doi.org/10.20944/preprints202411.0469.v1
Chicago/Turabian Style
Heatlie, J. K., Lisa M. Butler and Doug A. Brooks. 2024 "Prostate Cancer Intercellular Communication" Preprints. https://doi.org/10.20944/preprints202411.0469.v1
Abstract
Prostate cancer (PCa) pathogenesis relies on intercellular communication, which can involve tunneling nanotubes (TNTs) and extracellular vesicles (EVs). TNTs and EVs have been reported to transfer critical cargo involved in cellular function and signalling, prompting us to investigate the extent of organelle and protein transfer in PCa cells and the potential involvement of the androgen receptor. Using live cell imaging microscopy, we observed extensive formation of TNTs and EVs operating between PCa, non-malignant and immune cells. PCa cells were capable of transferring lysosomes, mitochondria, lipids and endoplasmic reticulum as well as syndecan-1, sortilin, Glut1 and Glut4. In mechanistic studies, androgen sensitive PCa cells exhibited changes in cell morphology when stimulated by R1881 treatment. Overexpression assays of a newly designed androgen receptor (AR) plasmid revealed its novel localization in PCa cellular vesicles, which were also transferred to neighbouring cells. Selected molecular machinery, thought to be involved in intercellular communication, was investigated by knockdown studies and Western blotting/immunofluorescence/scanning electron microscopy (SEM). PCa TNTs and EVs transported proteins and organelles, which may contain specialist signalling, programming and energy requirements that support cancer growth and progression. This makes these important intercellular communication systems ideal potential targets for therapeutic intervention.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
