Version 1
: Received: 6 November 2024 / Approved: 7 November 2024 / Online: 7 November 2024 (09:50:40 CET)
How to cite:
Laurel, S.; Gupta, K.; Nguyen, J.; Chandekar, A.; Le, J.; Berg, K.; Hirschberg, H. Enhancing the Efficacy of Radiation Therapy by Photochemical Internalization of Fibrin Hydrogel Delivered Bleomycin. Preprints2024, 2024110509. https://doi.org/10.20944/preprints202411.0509.v1
Laurel, S.; Gupta, K.; Nguyen, J.; Chandekar, A.; Le, J.; Berg, K.; Hirschberg, H. Enhancing the Efficacy of Radiation Therapy by Photochemical Internalization of Fibrin Hydrogel Delivered Bleomycin. Preprints 2024, 2024110509. https://doi.org/10.20944/preprints202411.0509.v1
Laurel, S.; Gupta, K.; Nguyen, J.; Chandekar, A.; Le, J.; Berg, K.; Hirschberg, H. Enhancing the Efficacy of Radiation Therapy by Photochemical Internalization of Fibrin Hydrogel Delivered Bleomycin. Preprints2024, 2024110509. https://doi.org/10.20944/preprints202411.0509.v1
APA Style
Laurel, S., Gupta, K., Nguyen, J., Chandekar, A., Le, J., Berg, K., & Hirschberg, H. (2024). Enhancing the Efficacy of Radiation Therapy by Photochemical Internalization of Fibrin Hydrogel Delivered Bleomycin. Preprints. https://doi.org/10.20944/preprints202411.0509.v1
Chicago/Turabian Style
Laurel, S., Kristian Berg and Henry Hirschberg. 2024 "Enhancing the Efficacy of Radiation Therapy by Photochemical Internalization of Fibrin Hydrogel Delivered Bleomycin" Preprints. https://doi.org/10.20944/preprints202411.0509.v1
Abstract
Background/Objectives: Although the use of radiation-sensitizing agents has been shown to enhance the effect of radiation on tumor cells, the blood-brain barrier (BBB) impedes these agents from reaching brain tumor sites when given systemically. Localized methods of sensitizer delivery, utilizing hydrogels, have the potential to bypass the blood-brain barrier. This study examined the ability of photochemical internalization (PCI) of hydrogel-released bleomycin to enhance the growth-inhibiting effects of radiation on multi-cell glioma spheroids in vitro; Methods: Loaded fibrin hydrogel layers were created by combining thrombin, fibrinogen, and bleomycin (BLM). Supernatants from these layers were collected, combined with photosensitizer, and added to F98 glioma spheroid cultures. Following light (PCI) and radiation treatment, at increasing dosages, spheroid growth was monitored for 14 days; Results: PCI of released BLM significantly reduced the radiation dose required to achieve equivalent efficacy compared to radiation or BLM+RT alone. Both immediate and delayed RT delivery post BLM-PCI resulted in similar degrees of growth inhibition; Conclusion: Non-degraded BLM was released from the fibrin hydrogel. PCI of BLM synergistically increased the growth-inhibiting effects of radiation treatment compared to radiation and BLM, and radiation acting as a single treatment.
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
