PreprintArticleVersion 1This version is not peer-reviewed
Simultaneous Down-regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-functionallized Gold Nanoprobes to Achieve Targeted Anti-tumor Therapy
Version 1
: Received: 6 November 2024 / Approved: 7 November 2024 / Online: 7 November 2024 (10:00:01 CET)
How to cite:
Ji, Q.; Yang, Q.; Ou, M.; Hong, M. Simultaneous Down-regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-functionallized Gold Nanoprobes to Achieve Targeted Anti-tumor Therapy. Preprints2024, 2024110511. https://doi.org/10.20944/preprints202411.0511.v1
Ji, Q.; Yang, Q.; Ou, M.; Hong, M. Simultaneous Down-regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-functionallized Gold Nanoprobes to Achieve Targeted Anti-tumor Therapy. Preprints 2024, 2024110511. https://doi.org/10.20944/preprints202411.0511.v1
Ji, Q.; Yang, Q.; Ou, M.; Hong, M. Simultaneous Down-regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-functionallized Gold Nanoprobes to Achieve Targeted Anti-tumor Therapy. Preprints2024, 2024110511. https://doi.org/10.20944/preprints202411.0511.v1
APA Style
Ji, Q., Yang, Q., Ou, M., & Hong, M. (2024). Simultaneous Down-regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-functionallized Gold Nanoprobes to Achieve Targeted Anti-tumor Therapy. Preprints. https://doi.org/10.20944/preprints202411.0511.v1
Chicago/Turabian Style
Ji, Q., Mengyao Ou and Min Hong. 2024 "Simultaneous Down-regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-functionallized Gold Nanoprobes to Achieve Targeted Anti-tumor Therapy" Preprints. https://doi.org/10.20944/preprints202411.0511.v1
Abstract
Telomerase presents over-expression in most of cancer cells and has been a near-universal marker of cancer. Studies revealed that inhibiting telomerase activity by utilizing oligonucleotides to down-regulate the expression of intracellular human telomerase reverse transcriptase (hTERT) mRNA was an effective method to achieve anti-tumor therapy. Considering oncogenic microRNA-21 has been proved to indirectly up-regulate hTERT expression and drive cancer metastasis and aggression through increased telomerase activity, here we constructed a AS1411-functionallized oligonucleotide-conjugated gold nanoprobe (Au-nanoprobe) to simultaneously down-regulate intracellular microRNA-21 and hTERT mRNA by using anti-sense oligonucleotide technology to explore their targeted anti-tumor therapy effect. In vitro cell studies demonstrated that Au-nanoprobes could effectively induce apoptosis and inhibit the proliferation of cancer cells through down-regulating intracellular hTERT activity. In vivo imaging and anti-tumor studies revealed that Au-nanoprobes could accumulate at the tumor site and inhibit the growth of MCF-7 tumor xenografted on balb/c nude mice, thus exert the potential of anti-tumor therapy.
Chemistry and Materials Science, Medicinal Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
