Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Laurel Forest Phyllosphere in the Canary Islands (Spain)

Nereida M. Rancel-Rodríguez; Nicole Sausen; Carolina Pérez Reyes; Antera Martel Quintana; Barbara Melkonian; Michael Melkonian

doi:10.20944/preprints202411.0515.v1

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preprints.org > biology and life sciences > plant sciences > doi: 10.20944/preprints202411.0515.v1 (registering DOI)

Preprint Article Version 1 This version is not peer-reviewed

Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Laurel Forest Phyllosphere in the Canary Islands (Spain)

Nereida M. Rancel-Rodríguez

^* ,

Nicole Sausen

Carolina Pérez Reyes

Antera Martel Quintana

Barbara Melkonian

Michael Melkonian

Version 1 : Received: 7 November 2024 / Approved: 7 November 2024 / Online: 8 November 2024 (02:17:16 CET)

How to cite: Rancel-Rodríguez, N. M.; Sausen, N.; Reyes, C. P.; Quintana, A. M.; Melkonian, B.; Melkonian, M. Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Laurel Forest Phyllosphere in the Canary Islands (Spain). Preprints 2024, 2024110515. https://doi.org/10.20944/preprints202411.0515.v1 Rancel-Rodríguez, N. M.; Sausen, N.; Reyes, C. P.; Quintana, A. M.; Melkonian, B.; Melkonian, M. Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Laurel Forest Phyllosphere in the Canary Islands (Spain). Preprints 2024, 2024110515. https://doi.org/10.20944/preprints202411.0515.v1

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MDPI and ACS Style

Rancel-Rodríguez, N. M.; Sausen, N.; Reyes, C. P.; Quintana, A. M.; Melkonian, B.; Melkonian, M. Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Laurel Forest Phyllosphere in the Canary Islands (Spain). Preprints 2024, 2024110515. https://doi.org/10.20944/preprints202411.0515.v1

APA Style

Rancel-Rodríguez, N. M., Sausen, N., Reyes, C. P., Quintana, A. M., Melkonian, B., & Melkonian, M. (2024). Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Laurel Forest Phyllosphere in the Canary Islands (Spain). Preprints. https://doi.org/10.20944/preprints202411.0515.v1

Chicago/Turabian Style

Rancel-Rodríguez, N. M., Barbara Melkonian and Michael Melkonian. 2024 "Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Laurel Forest Phyllosphere in the Canary Islands (Spain)" Preprints. https://doi.org/10.20944/preprints202411.0515.v1

Abstract

A total of 96 strains of Nostocales (Cyanobacteria) were established from the laurel forest phyllosphere in the Canary Islands (Spain) and Azores (Portugal) using enrichment media lacking combined nitrogen. The strains were characterized by light microscopy and SSU rRNA gene comparisons. Morphologically, most strains belonged to two different morphotypes, termed “Nostoc-type” and “Tolypothrix-type”. Molecular phylogenetic analysis of 527 SSU rRNA gene sequences of cyanobacteria (95 sequences established during this study plus 392 sequences from Nostocales and 40 sequences from non-heterocyte-forming cyanobacteria retrieved from the databases) revealed that none of the SSU rRNA gene sequences from the laurel forest phyllosphere was identical to a database sequence. In addition, the genetic diversity of the isolated strains was high with 42 different genotypes (44% of the sequences), recognized. Among the new genotypes were also terrestrial members of genus Nodularia as well as members of genus Brasilonema. It is concluded that heterocyte-forming cyanobacteria represent a still largely undersampled component of the phyllosphere in subtropical/tropical forests.

Keywords

biodiversity; clonal culture; epiphyllous; heterocyte-forming cyanobacteria; rRNA sequence comparison

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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