This article investigated Ta-Cu and Nb-Cu coatings deposited via magnetron sputtering from metal targets with varying copper content. Scanning electron microscopy (SEM) combined with energy dispersive spectroscopy (EDS) revealed that the coatings had an average thickness of 2.5 μm, with a Cu composition of approximately 10 wt.% and Ta exceeding 86%, with the remaining percentages attributed to carbon and oxygen from the residual vacuum chamber atmosphere. It was observed that increasing the coating thickness from 2.5 μm to 8.5 μm did not result in a significant change in nanohardness (3.29 GPa) in Nb-Cu coatings, although the elastic modulus increased by 15% (from 81 to 95 GPa). Ta-Cu coatings demonstrated superior antibacterial properties compared to Nb-Cu coatings. Consequently, Ta-Cu coatings exhibit considerable potential for further applications due to their favorable structural properties and enhanced antibacterial efficacy.