The increasing interest in developing silver nanoparticles as antibiotic raw materials has attracted much attention, as the most common reduction and electrolysis techniques produce the toxic gas byproduct nitrogen dioxide. This paper reports a successful effort to develop a modified toxic-free electrolysis technique to produce electrolytic silver nanoparticles (ESN). A comparison of the physical and biological properties of ESN and reductive silver nanoparticles (RSN) was made. The presence of silver atoms in the solution was determined using a UV visible spectrometer and absorption peaks were found at 425 nm (ESN) and 437 nm (RSN). The particle size in solution was determined using dynamic light scattering and the diameter was found to be approximately 40 nm (for ESN) and 70 nm (for RSN). Antibacterial efficacy and power to prevent the development of bacterial resistance against Propionibacterium acnes (P. acnes) were assessed using the Kirby-Bauer method. Statistical analysis of clear zone diameter data showed that unlike RSN, the efficacy of ESN increased with higher concentrations. The efficacy of ESN and RSN is relatively lower than Chloramphenicol 5% because it is measured in different concentration units (ESN and RSN in ppm and Chloramphenicol in %). By using a calibration curve, the efficacy of 5% Chloramphenicol can be equated to 0.005% ESN. In addition, P. acnes developed strong resistance to Chloramphenicol, weak resistance to RSN and showed no resistance to ESN. These findings underscore the extraordinary potential of ESN as a raw material for future antibiotics.