Background/Objective; In-vivo diabetes detection of glucose were sought using square-wave anodic stripping voltammetry (SW), with bismuth-immobilized carbon nanotube paste electrode (BCE). Methods:The optimum analytical results indicated sensitive peak signals on the BCE. The raw voltammogram was approached within the 1mgL-1-14mgL-1 and 10ugL-1-140 ugL-1, detection limits with preconcentration times of 100 and 200 sec. Results:The relative standard deviation was 0.02 % (n=15) of 10.0 mgL-1 under optimum conditions. The analytical detection limit (S/N) was attained at 8 ugL-1. The handmade microsensor was directly used in vivo on the living fish brain and human urine. Conclusion: The method was applied at real time in vivo, without requiring any pretreatment and other ionic electrolyte solutions. It can be used for medicinal and other materials requiring biological-fluid detection in real time. This study was designed to be suitable for real-time unmanned remote diagnosis and therapeutic drug injection into the body, micro-needle long-term administration, wearable artificial skin tattoo sensor, and real-time control. In addition, the glasses monitor was designed to be suitable for multitasking and multi-user control.