Solar eclipses present a valuable opportunity for controlled in-situ ionosphere studies. This work explores the response of the upper atmosphere’s F-layer during the total eclipse of April 8, 2024, which was primarily visible across North and South America. Employing a multi-instrument approach, we analyze the impact on the ionosphere’s Total Electron Content (TEC) and Very Low Frequency (VLF) signals over a three-day period encompassing the eclipse (April 7 to 9, 2024). Ground-based observations leverage data from ten strategically positioned International GNSS Service (IGS)/Global Positioning System (GPS) stations and four VLF stations situated along the eclipse path. We compute vertical TEC (VTEC) alongside temporal variations in VLF signal amplitude and phase to elucidate the ionosphere’s response. Notably, IGS station data reveal a decrease in VTEC during the partial and total solar eclipse phases, signifying a reduction in ionization. While VLF data also exhibit a general decrease, they display more prominent fluctuations. Space-based observations incorporate data from Swarm and COSMIC2 satellites as they traversed the eclipse path. Additionally, a spatiotemporal analysis utilizes data from the Global Ionospheric Map (GIM) database and the DLR’s (The German Aerospace Center’s) database. All space-based observations consistently demonstrate a significant depletion in VTEC during the eclipse. We further investigate the correlation between the percentage change in VTEC and the degree of solar obscuration, revealing a positive relationship. The consistent findings obtained from this comprehensive observational campaign bolster our understanding of the physical mechanisms governing ionospheric variability during solar eclipses. The observed depletion in VTEC align with the established principle that reduced solar radiation leads to decreased ionization within the ionosphere. Finally, geomagnetic data analysis confirms that external disturbances did not significantly influence our observations.