Human Parvovirus B19 (B19V) is a widespread virus, causing erythema infectiosum in children and several clinical manifestations from acute to persistent infections in adults. In early 2024, ECDC reported an increased human parvovirus B19 circulation in 14 European countries. A Parvovirus B19 outbreak was also reported in Tuscany, Italy, prompting a detailed investigation of its genetic characteristics. This study is the first Italian report on this outbreak, which carries out a phylogenetic analysis based on the complete coding B19V genomes of 23 positive samples. Phylogenetic clustering assigned all isolates to the G1a genotype, although with some mutations in NS1, VP1 and VP2, compared to the Reference strains.